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Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles
dc.creator | Dorm, Bruna Carolina | |
dc.creator | Rosas Costa Iemma, Mônica | |
dc.creator | Domingos Neto, Benedito | |
dc.creator | Lopes Francisco, Rauany Cristina | |
dc.creator | Dinić, Ivana | |
dc.creator | Ignjatović, Nenad | |
dc.creator | Marković, Smilja | |
dc.creator | Vuković, Marina | |
dc.creator | Škapin, Srečo | |
dc.creator | Trovatti, Eliane | |
dc.creator | Mančić, Lidija | |
dc.date.accessioned | 2023-03-01T11:20:20Z | |
dc.date.available | 2023-03-01T11:20:20Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2075-1729 | |
dc.identifier.uri | http://cherry.chem.bg.ac.rs/handle/123456789/5814 | |
dc.description.abstract | Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine. | |
dc.language | en | |
dc.publisher | MDPI | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200175/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200288/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Life | |
dc.subject | alanine | |
dc.subject | amino acid | |
dc.subject | cell adhesion | |
dc.subject | cell viability | |
dc.subject | hydroxyapatite | |
dc.subject | surface modification | |
dc.title | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles | |
dc.type | article | en |
dc.rights.license | BY | |
dc.citation.volume | 13 | |
dc.citation.issue | 1 | |
dc.citation.spage | 116 | |
dc.identifier.doi | 10.3390/life13010116 | |
dc.citation.rank | M22~ | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85146759973 | |
dc.identifier.fulltext | http://cherry.chem.bg.ac.rs/bitstream/id/32517/Synthesis_and_Biological_pub_2023.pdf |