Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
Authors
Jurič, AndrejaBrčić Karačonji, Irena

Gašić, Uroš M.

Milojković-Opsenica, Dušanka

Prđun, Saša
Bubalo, Dragan
Lušić, Dražen

Vahčić, Nada
Kopjar, Nevenka

Dataset (Published version)
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Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were establis...hed. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
Keywords:
Arbutus unedo / chromosome aberration analysis / cytokinesis-block micronucleus cytome assay / honey / irinotecan / lymphocytes / phenolics / UHPLC-LTQ Orbitrap MSSource:
International Journal of Molecular Sciences, 2023, 24, 3Publisher:
- MDPI
Funding / projects:
- Croatian Academy of Sciences and Arts and institutional funding provided by the Ministry of Science and Education of the Republic of Croatia
Note:
- Related to published version: https://cherry.chem.bg.ac.rs/handle/123456789/5874
- Supplementary material for: https://doi.org/10.3390/ijms24031903
Related info:
- Referenced by
https://doi.org/10.3390/ijms24031903 - Referenced by
https://cherry.chem.bg.ac.rs/handle/123456789/5874
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Hemijski fakultet / Faculty of ChemistryTY - DATA AU - Jurič, Andreja AU - Brčić Karačonji, Irena AU - Gašić, Uroš M. AU - Milojković-Opsenica, Dušanka AU - Prđun, Saša AU - Bubalo, Dragan AU - Lušić, Dražen AU - Vahčić, Nada AU - Kopjar, Nevenka PY - 2023 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5875 AB - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models. PB - MDPI T2 - International Journal of Molecular Sciences T1 - Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study VL - 24 IS - 3 UR - https://hdl.handle.net/21.15107/rcub_cherry_5875 ER -
@misc{ author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka", year = "2023", abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study", volume = "24", number = "3", url = "https://hdl.handle.net/21.15107/rcub_cherry_5875" }
Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences MDPI., 24(3). https://hdl.handle.net/21.15107/rcub_cherry_5875
Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3). https://hdl.handle.net/21.15107/rcub_cherry_5875 .
Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023), https://hdl.handle.net/21.15107/rcub_cherry_5875 .