Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin
Конференцијски прилог (Објављена верзија)
Метаподаци
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Food allergies represent important health problem in industrialized countries, with seafood being recognized as one of the 8 most common sources of allergens. While there are several proteins that have been linked to shellfish allergy, tropomyosin accounts for majority of diagnozed ingestion-related shellfish allergies. Presence of even traces of allergens in food can be a serious health hazard to consumers, which is why proper labeling of food products by food manufacturers is of critical importance for sensitized persons. On the other hand, development of reliable, specific and sensitive methods for detection and quantification of allergens in food products is of the high importance as well. The objective of this study was to develop highly sensitive immuno-polymerase chain reaction (immuno PCR) method for the detection and quantification of shellfish tropomyosin in food samples. Immuno PCR method couples standard sandwich enzyme-linked immunosorbent assay (ELISA) format with real ti...me PCR. Monoclonal antibody was used as capture antibody, while biotinylated polyclonal antibody served as detection antibody. Reporter biotinylated DNA was coupled to detection antibody via streptavidin and subsequently amplified and quantified by real time PCR. Tropomyosin was quantified using highly purified natural shrimp tropomyosin as standard. The results were compared to standard sandwich ELISA.
Извор:
2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts, 2020, S4-S4Издавач:
- University of Vienna
Финансирање / пројекти:
- Молекуларне особине и модификације неких респираторних и нутритивних алергена (RS-MESTD-Basic Research (BR or ON)-172024)
- FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics (EU-H2020-810752)
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Radomirović, Mirjana Ž. AU - Ćirković-Veličković, Tanja AU - Rajković, Andreja PY - 2020 UR - http://cherry.chem.bg.ac.rs/handle/123456789/6036 AB - Food allergies represent important health problem in industrialized countries, with seafood being recognized as one of the 8 most common sources of allergens. While there are several proteins that have been linked to shellfish allergy, tropomyosin accounts for majority of diagnozed ingestion-related shellfish allergies. Presence of even traces of allergens in food can be a serious health hazard to consumers, which is why proper labeling of food products by food manufacturers is of critical importance for sensitized persons. On the other hand, development of reliable, specific and sensitive methods for detection and quantification of allergens in food products is of the high importance as well. The objective of this study was to develop highly sensitive immuno-polymerase chain reaction (immuno PCR) method for the detection and quantification of shellfish tropomyosin in food samples. Immuno PCR method couples standard sandwich enzyme-linked immunosorbent assay (ELISA) format with real time PCR. Monoclonal antibody was used as capture antibody, while biotinylated polyclonal antibody served as detection antibody. Reporter biotinylated DNA was coupled to detection antibody via streptavidin and subsequently amplified and quantified by real time PCR. Tropomyosin was quantified using highly purified natural shrimp tropomyosin as standard. The results were compared to standard sandwich ELISA. PB - University of Vienna C3 - 2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts T1 - Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin SP - S4 EP - S4 UR - https://hdl.handle.net/21.15107/rcub_cherry_6036 ER -
@conference{ author = "Radomirović, Mirjana Ž. and Ćirković-Veličković, Tanja and Rajković, Andreja", year = "2020", abstract = "Food allergies represent important health problem in industrialized countries, with seafood being recognized as one of the 8 most common sources of allergens. While there are several proteins that have been linked to shellfish allergy, tropomyosin accounts for majority of diagnozed ingestion-related shellfish allergies. Presence of even traces of allergens in food can be a serious health hazard to consumers, which is why proper labeling of food products by food manufacturers is of critical importance for sensitized persons. On the other hand, development of reliable, specific and sensitive methods for detection and quantification of allergens in food products is of the high importance as well. The objective of this study was to develop highly sensitive immuno-polymerase chain reaction (immuno PCR) method for the detection and quantification of shellfish tropomyosin in food samples. Immuno PCR method couples standard sandwich enzyme-linked immunosorbent assay (ELISA) format with real time PCR. Monoclonal antibody was used as capture antibody, while biotinylated polyclonal antibody served as detection antibody. Reporter biotinylated DNA was coupled to detection antibody via streptavidin and subsequently amplified and quantified by real time PCR. Tropomyosin was quantified using highly purified natural shrimp tropomyosin as standard. The results were compared to standard sandwich ELISA.", publisher = "University of Vienna", journal = "2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts", title = "Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin", pages = "S4-S4", url = "https://hdl.handle.net/21.15107/rcub_cherry_6036" }
Radomirović, M. Ž., Ćirković-Veličković, T.,& Rajković, A.. (2020). Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin. in 2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts University of Vienna., S4-S4. https://hdl.handle.net/21.15107/rcub_cherry_6036
Radomirović MŽ, Ćirković-Veličković T, Rajković A. Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin. in 2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts. 2020;:S4-S4. https://hdl.handle.net/21.15107/rcub_cherry_6036 .
Radomirović, Mirjana Ž., Ćirković-Veličković, Tanja, Rajković, Andreja, "Development of an immuno-polymerase chain reaction for detection and quantification of shellfish tropomyosin" in 2nd FoodEnTwin Workshop “Experimental animal models for food and environment”, Vienna, Austria, 3rd-4th February, 2020. In: Book of Abstracts (2020):S4-S4, https://hdl.handle.net/21.15107/rcub_cherry_6036 .