Salting-out thin-layer chromatography of some macrolide antibiotics

Abstract

The chromatographic behavior of roxithromycin, midecamycin, erythromycin, azithromycin, and erythromycin ethylsuccinate has been studied by salting-out thin-layer chromatography (SOTLC) with cellulose as a adsorbent and aqueous ammonium sulfate solutions of different concentration as mobile phases. Hydrophobicity was determined from the linear relationships between solute R-M values and the ammonium sulfate content of the mobile phase. Hydrophobicity values R-M(0) and C-0 were calculated on the basis of the results obtained. Lipophilicity determined in this way was correlated with calculated log P values.