Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species
Нема приказа
Аутори
Chuck, Jo-AnneDunn, Catherine
Facultad, Fe E. C. D.
Nakazono, Chojin
Nikodinović-Runić, Jasmina
Barrow, Kevin D.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.
Извор:
Current Microbiology, 2006, 53, 2, 89-94Издавач:
- Springer, New York
DOI: 10.1007/s00284-005-0050-x
ISSN: 0343-8651
PubMed: 16832727
WoS: 000239085000001
Scopus: 2-s2.0-33746729478
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Chuck, Jo-Anne AU - Dunn, Catherine AU - Facultad, Fe E. C. D. AU - Nakazono, Chojin AU - Nikodinović-Runić, Jasmina AU - Barrow, Kevin D. PY - 2006 UR - https://cherry.chem.bg.ac.rs/handle/123456789/786 AB - Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources. PB - Springer, New York T2 - Current Microbiology T1 - Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species VL - 53 IS - 2 SP - 89 EP - 94 DO - 10.1007/s00284-005-0050-x ER -
@article{ author = "Chuck, Jo-Anne and Dunn, Catherine and Facultad, Fe E. C. D. and Nakazono, Chojin and Nikodinović-Runić, Jasmina and Barrow, Kevin D.", year = "2006", abstract = "Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.", publisher = "Springer, New York", journal = "Current Microbiology", title = "Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species", volume = "53", number = "2", pages = "89-94", doi = "10.1007/s00284-005-0050-x" }
Chuck, J., Dunn, C., Facultad, F. E. C. D., Nakazono, C., Nikodinović-Runić, J.,& Barrow, K. D.. (2006). Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species. in Current Microbiology Springer, New York., 53(2), 89-94. https://doi.org/10.1007/s00284-005-0050-x
Chuck J, Dunn C, Facultad FECD, Nakazono C, Nikodinović-Runić J, Barrow KD. Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species. in Current Microbiology. 2006;53(2):89-94. doi:10.1007/s00284-005-0050-x .
Chuck, Jo-Anne, Dunn, Catherine, Facultad, Fe E. C. D., Nakazono, Chojin, Nikodinović-Runić, Jasmina, Barrow, Kevin D., "Amplification of DNA encoding entire type I polyketide synthase domains and linkers from Streptomyces species" in Current Microbiology, 53, no. 2 (2006):89-94, https://doi.org/10.1007/s00284-005-0050-x . .