Bilirubin determination in gallstones
Abstract
The determination of bilirubin in gallstones is carried out by means of several methods, but most frequently by spectrophotometric determination of azobilirubin. We found that most of the methods were not applicable to bilirubin determination in certain types of gallstones. Thus, we developed a simple and suitable method for bilirubin determination applicable to all gallstone specimens. Based on direct spectrophotometric measurements of chloroform solution of bilirubin or gallstone extracts, our method was found to be sensitive enough to determine small amounts as well as high contents of bilirubin in gallstones. Other gallstone components were of no influence on the assay; thus this method proved to be superior to azobilirubin or other assays. © 1991 Springer-Verlag.
Source:
Fresenius' Journal of Analytical Chemistry, 1991, 341, 12, 723-726Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Todorović, Marija B. AU - Djurdjic, V. AU - Mandić, Ljuba M. PY - 1991 UR - https://cherry.chem.bg.ac.rs/handle/123456789/79 AB - The determination of bilirubin in gallstones is carried out by means of several methods, but most frequently by spectrophotometric determination of azobilirubin. We found that most of the methods were not applicable to bilirubin determination in certain types of gallstones. Thus, we developed a simple and suitable method for bilirubin determination applicable to all gallstone specimens. Based on direct spectrophotometric measurements of chloroform solution of bilirubin or gallstone extracts, our method was found to be sensitive enough to determine small amounts as well as high contents of bilirubin in gallstones. Other gallstone components were of no influence on the assay; thus this method proved to be superior to azobilirubin or other assays. © 1991 Springer-Verlag. T2 - Fresenius' Journal of Analytical Chemistry T1 - Bilirubin determination in gallstones VL - 341 IS - 12 SP - 723 EP - 726 DO - 10.1007/BF00321575 ER -
@article{ author = "Todorović, Marija B. and Djurdjic, V. and Mandić, Ljuba M.", year = "1991", abstract = "The determination of bilirubin in gallstones is carried out by means of several methods, but most frequently by spectrophotometric determination of azobilirubin. We found that most of the methods were not applicable to bilirubin determination in certain types of gallstones. Thus, we developed a simple and suitable method for bilirubin determination applicable to all gallstone specimens. Based on direct spectrophotometric measurements of chloroform solution of bilirubin or gallstone extracts, our method was found to be sensitive enough to determine small amounts as well as high contents of bilirubin in gallstones. Other gallstone components were of no influence on the assay; thus this method proved to be superior to azobilirubin or other assays. © 1991 Springer-Verlag.", journal = "Fresenius' Journal of Analytical Chemistry", title = "Bilirubin determination in gallstones", volume = "341", number = "12", pages = "723-726", doi = "10.1007/BF00321575" }
Todorović, M. B., Djurdjic, V.,& Mandić, L. M.. (1991). Bilirubin determination in gallstones. in Fresenius' Journal of Analytical Chemistry, 341(12), 723-726. https://doi.org/10.1007/BF00321575
Todorović MB, Djurdjic V, Mandić LM. Bilirubin determination in gallstones. in Fresenius' Journal of Analytical Chemistry. 1991;341(12):723-726. doi:10.1007/BF00321575 .
Todorović, Marija B., Djurdjic, V., Mandić, Ljuba M., "Bilirubin determination in gallstones" in Fresenius' Journal of Analytical Chemistry, 341, no. 12 (1991):723-726, https://doi.org/10.1007/BF00321575 . .