Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and cor...related with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo. (c) 2006 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Кључне речи:
cholesterol / phospholipids / haemoglobin / red blood cells / HDL cholesterol / lipoproteinsИзвор:
Clinical Biochemistry, 2007, 40, 5-6, 305-309Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Хемијске и биохемијске консеквенце метал-лиганд интеракција, II. део (RS-MESTD-MPN2006-2010-142017)
DOI: 10.1016/j.clinbiochem.2006.11.005
ISSN: 0009-9120
PubMed: 17291471
WoS: 000244997400004
Scopus: 2-s2.0-33847313315
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Nikolić, Milan AU - Stanić, Dragana AU - Barievic, Ivona AU - Jones, David R. AU - Nedić, Olgica AU - Niketić, Vesna PY - 2007 UR - https://cherry.chem.bg.ac.rs/handle/123456789/824 AB - Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo. (c) 2006 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Clinical Biochemistry T1 - Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma VL - 40 IS - 5-6 SP - 305 EP - 309 DO - 10.1016/j.clinbiochem.2006.11.005 ER -
@article{ author = "Nikolić, Milan and Stanić, Dragana and Barievic, Ivona and Jones, David R. and Nedić, Olgica and Niketić, Vesna", year = "2007", abstract = "Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo. (c) 2006 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Clinical Biochemistry", title = "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma", volume = "40", number = "5-6", pages = "305-309", doi = "10.1016/j.clinbiochem.2006.11.005" }
Nikolić, M., Stanić, D., Barievic, I., Jones, D. R., Nedić, O.,& Niketić, V.. (2007). Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry Pergamon-Elsevier Science Ltd, Oxford., 40(5-6), 305-309. https://doi.org/10.1016/j.clinbiochem.2006.11.005
Nikolić M, Stanić D, Barievic I, Jones DR, Nedić O, Niketić V. Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry. 2007;40(5-6):305-309. doi:10.1016/j.clinbiochem.2006.11.005 .
Nikolić, Milan, Stanić, Dragana, Barievic, Ivona, Jones, David R., Nedić, Olgica, Niketić, Vesna, "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma" in Clinical Biochemistry, 40, no. 5-6 (2007):305-309, https://doi.org/10.1016/j.clinbiochem.2006.11.005 . .