Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method
Само за регистроване кориснике
2007
Аутори
Blanusa, MilanPerovic, Iva
Popović, Milica
Polović, Natalija
Burazer, Lidija M.
Milovanovic, Mina
Gavrović-Jankulović, Marija
Jankov, Ratko M.
Ćirković-Veličković, Tanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved.
Кључне речи:
actinidin / act c 1 / allergen / art v 1 / ion-exchange / HPLC / quantification / kiwi fruit / mugwort / pollenИзвор:
Journal of Chromatography B: Analytical Technologies in the Biomedical and L, 2007, 857, 2, 188-194Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-MESTD-MPN2006-2010-142020)
DOI: 10.1016/j.jchromb.2007.07.015
ISSN: 1570-0232
PubMed: 17652037
WoS: 000250211500002
Scopus: 2-s2.0-34548823476
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Blanusa, Milan AU - Perovic, Iva AU - Popović, Milica AU - Polović, Natalija AU - Burazer, Lidija M. AU - Milovanovic, Mina AU - Gavrović-Jankulović, Marija AU - Jankov, Ratko M. AU - Ćirković-Veličković, Tanja PY - 2007 UR - https://cherry.chem.bg.ac.rs/handle/123456789/885 AB - A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Chromatography B: Analytical Technologies in the Biomedical and L T1 - Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method VL - 857 IS - 2 SP - 188 EP - 194 DO - 10.1016/j.jchromb.2007.07.015 ER -
@article{ author = "Blanusa, Milan and Perovic, Iva and Popović, Milica and Polović, Natalija and Burazer, Lidija M. and Milovanovic, Mina and Gavrović-Jankulović, Marija and Jankov, Ratko M. and Ćirković-Veličković, Tanja", year = "2007", abstract = "A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and L", title = "Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method", volume = "857", number = "2", pages = "188-194", doi = "10.1016/j.jchromb.2007.07.015" }
Blanusa, M., Perovic, I., Popović, M., Polović, N., Burazer, L. M., Milovanovic, M., Gavrović-Jankulović, M., Jankov, R. M.,& Ćirković-Veličković, T.. (2007). Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method. in Journal of Chromatography B: Analytical Technologies in the Biomedical and L Elsevier Science Bv, Amsterdam., 857(2), 188-194. https://doi.org/10.1016/j.jchromb.2007.07.015
Blanusa M, Perovic I, Popović M, Polović N, Burazer LM, Milovanovic M, Gavrović-Jankulović M, Jankov RM, Ćirković-Veličković T. Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method. in Journal of Chromatography B: Analytical Technologies in the Biomedical and L. 2007;857(2):188-194. doi:10.1016/j.jchromb.2007.07.015 .
Blanusa, Milan, Perovic, Iva, Popović, Milica, Polović, Natalija, Burazer, Lidija M., Milovanovic, Mina, Gavrović-Jankulović, Marija, Jankov, Ratko M., Ćirković-Veličković, Tanja, "Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method" in Journal of Chromatography B: Analytical Technologies in the Biomedical and L, 857, no. 2 (2007):188-194, https://doi.org/10.1016/j.jchromb.2007.07.015 . .