Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells
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Ćirković-Veličković, TanjaThunberg, Sarah
Polović, Natalija
Neimert-Andersson, Theresa
Gronlund, Hans
van Hage, Marianne
Gafvelin, Guro
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Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS/mg protein). PBMCs prepared from 15 subjects having IgE to cat ( gt 0.7 kU(A)/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 mu g/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFN gamma, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-pos...itive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 mu g/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFN gamma in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro. Copyright (C) 2007 S. Karger AG, Basel.
Keywords:
cat allergen / Fel d 1 / endotoxin / lipopolysaccharide T cell proliferationSource:
International Archives of Allergy and Immunology, 2008, 146, 1, 1-10Publisher:
- Karger, Basel
DOI: 10.1159/000112497
ISSN: 1018-2438
PubMed: 18087156
WoS: 000255174700002
Scopus: 2-s2.0-42449125706
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ćirković-Veličković, Tanja AU - Thunberg, Sarah AU - Polović, Natalija AU - Neimert-Andersson, Theresa AU - Gronlund, Hans AU - van Hage, Marianne AU - Gafvelin, Guro PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/934 AB - Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS/mg protein). PBMCs prepared from 15 subjects having IgE to cat ( gt 0.7 kU(A)/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 mu g/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFN gamma, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-positive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 mu g/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFN gamma in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro. Copyright (C) 2007 S. Karger AG, Basel. PB - Karger, Basel T2 - International Archives of Allergy and Immunology T1 - Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells VL - 146 IS - 1 SP - 1 EP - 10 DO - 10.1159/000112497 ER -
@article{ author = "Ćirković-Veličković, Tanja and Thunberg, Sarah and Polović, Natalija and Neimert-Andersson, Theresa and Gronlund, Hans and van Hage, Marianne and Gafvelin, Guro", year = "2008", abstract = "Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS/mg protein). PBMCs prepared from 15 subjects having IgE to cat ( gt 0.7 kU(A)/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 mu g/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFN gamma, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-positive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 mu g/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFN gamma in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro. Copyright (C) 2007 S. Karger AG, Basel.", publisher = "Karger, Basel", journal = "International Archives of Allergy and Immunology", title = "Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells", volume = "146", number = "1", pages = "1-10", doi = "10.1159/000112497" }
Ćirković-Veličković, T., Thunberg, S., Polović, N., Neimert-Andersson, T., Gronlund, H., van Hage, M.,& Gafvelin, G.. (2008). Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells. in International Archives of Allergy and Immunology Karger, Basel., 146(1), 1-10. https://doi.org/10.1159/000112497
Ćirković-Veličković T, Thunberg S, Polović N, Neimert-Andersson T, Gronlund H, van Hage M, Gafvelin G. Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells. in International Archives of Allergy and Immunology. 2008;146(1):1-10. doi:10.1159/000112497 .
Ćirković-Veličković, Tanja, Thunberg, Sarah, Polović, Natalija, Neimert-Andersson, Theresa, Gronlund, Hans, van Hage, Marianne, Gafvelin, Guro, "Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells" in International Archives of Allergy and Immunology, 146, no. 1 (2008):1-10, https://doi.org/10.1159/000112497 . .