Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA
Само за регистроване кориснике
2008
Аутори
Gavrović-Jankulović, MarijaSpasic, Milena
Ćirković-Veličković, Tanja
Stojanović, Marijana M.
Inić-Kanada, Aleksandra
Dimitrijevic, Ljiliana
Lindner, Buko
Petersen, Arnd
Becker, Wolf-Meinhard
Jankov, Ratko M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwi fruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the e...valuation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis.
Кључне речи:
Act d 2 / food allergy / immunoassay / kiwifruit / thaumatin-like proteinИзвор:
Molecular Nutrition and Food Research, 2008, 52, 6, 701-707Издавач:
- Wiley-Blackwell, Malden
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-142020)
DOI: 10.1002/mnfr.200700286
ISSN: 1613-4125
PubMed: 18384094
WoS: 000256983900008
Scopus: 2-s2.0-49649110716
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gavrović-Jankulović, Marija AU - Spasic, Milena AU - Ćirković-Veličković, Tanja AU - Stojanović, Marijana M. AU - Inić-Kanada, Aleksandra AU - Dimitrijevic, Ljiliana AU - Lindner, Buko AU - Petersen, Arnd AU - Becker, Wolf-Meinhard AU - Jankov, Ratko M. PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/948 AB - Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwi fruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the evaluation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis. PB - Wiley-Blackwell, Malden T2 - Molecular Nutrition and Food Research T1 - Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA VL - 52 IS - 6 SP - 701 EP - 707 DO - 10.1002/mnfr.200700286 ER -
@article{ author = "Gavrović-Jankulović, Marija and Spasic, Milena and Ćirković-Veličković, Tanja and Stojanović, Marijana M. and Inić-Kanada, Aleksandra and Dimitrijevic, Ljiliana and Lindner, Buko and Petersen, Arnd and Becker, Wolf-Meinhard and Jankov, Ratko M.", year = "2008", abstract = "Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwi fruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the evaluation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis.", publisher = "Wiley-Blackwell, Malden", journal = "Molecular Nutrition and Food Research", title = "Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA", volume = "52", number = "6", pages = "701-707", doi = "10.1002/mnfr.200700286" }
Gavrović-Jankulović, M., Spasic, M., Ćirković-Veličković, T., Stojanović, M. M., Inić-Kanada, A., Dimitrijevic, L., Lindner, B., Petersen, A., Becker, W.,& Jankov, R. M.. (2008). Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA. in Molecular Nutrition and Food Research Wiley-Blackwell, Malden., 52(6), 701-707. https://doi.org/10.1002/mnfr.200700286
Gavrović-Jankulović M, Spasic M, Ćirković-Veličković T, Stojanović MM, Inić-Kanada A, Dimitrijevic L, Lindner B, Petersen A, Becker W, Jankov RM. Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA. in Molecular Nutrition and Food Research. 2008;52(6):701-707. doi:10.1002/mnfr.200700286 .
Gavrović-Jankulović, Marija, Spasic, Milena, Ćirković-Veličković, Tanja, Stojanović, Marijana M., Inić-Kanada, Aleksandra, Dimitrijevic, Ljiliana, Lindner, Buko, Petersen, Arnd, Becker, Wolf-Meinhard, Jankov, Ratko M., "Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA" in Molecular Nutrition and Food Research, 52, no. 6 (2008):701-707, https://doi.org/10.1002/mnfr.200700286 . .