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Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA
dc.creator | Gavrović-Jankulović, Marija | |
dc.creator | Spasic, Milena | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.creator | Stojanović, Marijana M. | |
dc.creator | Inić-Kanada, Aleksandra | |
dc.creator | Dimitrijevic, Ljiliana | |
dc.creator | Lindner, Buko | |
dc.creator | Petersen, Arnd | |
dc.creator | Becker, Wolf-Meinhard | |
dc.creator | Jankov, Ratko M. | |
dc.date.accessioned | 2018-11-22T00:13:13Z | |
dc.date.available | 2018-11-22T00:13:13Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1613-4125 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/948 | |
dc.description.abstract | Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwi fruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the evaluation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis. | en |
dc.publisher | Wiley-Blackwell, Malden | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS// | |
dc.rights | restrictedAccess | |
dc.source | Molecular Nutrition and Food Research | |
dc.subject | Act d 2 | en |
dc.subject | food allergy | en |
dc.subject | immunoassay | en |
dc.subject | kiwifruit | en |
dc.subject | thaumatin-like protein | en |
dc.title | Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ћирковић-Величковић, Тања; Спасиц, Милена; Петерсен, Aрнд; Иниц-Канада, Aлександра; Гавровић-Јанкуловић, Марија; Јанков, Ратко М.; Стојановиц, Маријана; Линднер, Буко; Бецкер, Wолф-Меинхард; Димитријевиц, Љилиана; | |
dc.citation.volume | 52 | |
dc.citation.issue | 6 | |
dc.citation.spage | 701 | |
dc.citation.epage | 707 | |
dc.identifier.wos | 000256983900008 | |
dc.identifier.doi | 10.1002/mnfr.200700286 | |
dc.citation.other | 52(6): 701-707 | |
dc.citation.rank | aM21 | |
dc.identifier.pmid | 18384094 | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-49649110716 |