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Antioxidant properties of grape seed extract on human lymphocyte oxidative defence

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Authors
Stanković, Miroslava
Tešević, Vele
Vajs, Vlatka
Todorović, Nina
Milosavljević, Slobodan M.
Gođevac, Dejan
Article (Published version)
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Abstract
The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocy...te damage by ROS.

Keywords:
Vitis vinifera / Vitaceae / micronucleus test / antioxidant enzymes / human lymphocytes / LC/MS / NMR
Source:
Planta Medica, 2008, 74, 7, 730-735
Publisher:
  • Georg Thieme Verlag Kg, Stuttgart

DOI: 10.1055/s-2008-1074521

ISSN: 0032-0943

PubMed: 18496781

WoS: 000257284700005

Scopus: 2-s2.0-45849128011
[ Google Scholar ]
36
24
URI
https://cherry.chem.bg.ac.rs/handle/123456789/952
Collections
  • Publikacije
Institution/Community
Hemijski fakultet
TY  - JOUR
AU  - Stanković, Miroslava
AU  - Tešević, Vele
AU  - Vajs, Vlatka
AU  - Todorović, Nina
AU  - Milosavljević, Slobodan M.
AU  - Gođevac, Dejan
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/952
AB  - The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.
PB  - Georg Thieme Verlag Kg, Stuttgart
T2  - Planta Medica
T1  - Antioxidant properties of grape seed extract on human lymphocyte oxidative defence
VL  - 74
IS  - 7
SP  - 730
EP  - 735
DO  - 10.1055/s-2008-1074521
UR  - Kon_1905
ER  - 
@article{
author = "Stanković, Miroslava and Tešević, Vele and Vajs, Vlatka and Todorović, Nina and Milosavljević, Slobodan M. and Gođevac, Dejan",
year = "2008",
abstract = "The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence",
volume = "74",
number = "7",
pages = "730-735",
doi = "10.1055/s-2008-1074521",
url = "Kon_1905"
}
Stanković, M., Tešević, V., Vajs, V., Todorović, N., Milosavljević, S. M.,& Gođevac, D.. (2008). Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 74(7), 730-735.
https://doi.org/10.1055/s-2008-1074521
Kon_1905
Stanković M, Tešević V, Vajs V, Todorović N, Milosavljević SM, Gođevac D. Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica. 2008;74(7):730-735.
doi:10.1055/s-2008-1074521
Kon_1905 .
Stanković, Miroslava, Tešević, Vele, Vajs, Vlatka, Todorović, Nina, Milosavljević, Slobodan M., Gođevac, Dejan, "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence" in Planta Medica, 74, no. 7 (2008):730-735,
https://doi.org/10.1055/s-2008-1074521 .,
Kon_1905 .

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