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dc.creatorMartin, Leona B.
dc.creatorNikodinović-Runić, Jasmina
dc.creatorMcMahon, Aoife M.
dc.creatorVijgenboom, Erik
dc.creatorO'Connor, Kevin E.
dc.date.accessioned2018-11-22T00:13:25Z
dc.date.available2018-11-22T00:13:25Z
dc.date.issued2008
dc.identifier.issn0141-0229
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/960
dc.description.abstractTyrosinase (tyr) purified from Pseudomonas putida F6, Streptomyces antibioticus, and Agaricus bisporus (mushroom) oxidises 3 regioisomers of fluorophenol as well as 3,4-difluorophenol (3,4FP). The catalytic efficiency (k(cat)/K-m) of tyrosinase towards any one substrate is different for each enzyme source. Oftentimes a large difference in affinity (K-m), and turnover (k(cat)) is observed for different enzyme sources with the same substrate. The best catalytic efficiency towards a fluorinated substrate was observed for P putida F6 tyr with 4-fluorophenol (4FP). The presence of a second fluorine on the aromatic ring (3,4FP) resulted in a decrease in the catalytic efficiency of all three enzymes compared to values for 4FP. However, the Km value for 3,4FP decreased for P. putida F6 tyr indicating a higher affinity for P. putida F6 tyr for 3,4FP compared to 4FP. Furthermore the k(cat) value for 3,4FP increased for mushroom tyr in comparison to the value for 4FP indicating a higher maximum turnover of 3,4FP compared to 4FP for mushroom tyr. All three sources of tyr exhibited lower catalytic efficiencies for 3-fluorophenol (3FP) and 2-fluorophenol (2FP) compared to 4FP. However, the K-m value for 2FP was lower than that for 3FP for both S. antibioticus and mushroom tyr indicating a higher affinity for 2FP over 3FP. (C) 2008 Elsevier Inc. All rights reserved.en
dc.publisherElsevier Science Inc, New York
dc.rightsrestrictedAccess
dc.sourceEnzyme and Microbial Technology
dc.subjectPseudomonas putida F6en
dc.subjectStreptomyces antibioticusen
dc.subjectAgaricus bisporus (mushroom)en
dc.subjecttyrosinaseen
dc.subject4-fluorophenolen
dc.subject3,4-difluorophenolen
dc.subject3-fluorophenolen
dc.subject2-fluorophenolen
dc.titleAssessing the catalytic activity of three different sources of tyrosinase: A study of the oxidation of mono- and difluorinated monophenolsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМартин, Леона Б.; Вијгенбоом, Ерик; Никодиновић-Рунић, Јасмина; МцМахон, Aоифе М.; О'Цоннор, Кевин Е.;
dc.citation.volume43
dc.citation.issue3
dc.citation.spage297
dc.citation.epage301
dc.identifier.wos000258217800011
dc.identifier.doi10.1016/j.enzmictec.2008.03.010
dc.citation.other43(3): 297-301
dc.citation.rankM22
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-46749111362


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