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Assessing the catalytic activity of three different sources of tyrosinase: A study of the oxidation of mono- and difluorinated monophenols
dc.creator | Martin, Leona B. | |
dc.creator | Nikodinović-Runić, Jasmina | |
dc.creator | McMahon, Aoife M. | |
dc.creator | Vijgenboom, Erik | |
dc.creator | O'Connor, Kevin E. | |
dc.date.accessioned | 2018-11-22T00:13:25Z | |
dc.date.available | 2018-11-22T00:13:25Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0141-0229 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/960 | |
dc.description.abstract | Tyrosinase (tyr) purified from Pseudomonas putida F6, Streptomyces antibioticus, and Agaricus bisporus (mushroom) oxidises 3 regioisomers of fluorophenol as well as 3,4-difluorophenol (3,4FP). The catalytic efficiency (k(cat)/K-m) of tyrosinase towards any one substrate is different for each enzyme source. Oftentimes a large difference in affinity (K-m), and turnover (k(cat)) is observed for different enzyme sources with the same substrate. The best catalytic efficiency towards a fluorinated substrate was observed for P putida F6 tyr with 4-fluorophenol (4FP). The presence of a second fluorine on the aromatic ring (3,4FP) resulted in a decrease in the catalytic efficiency of all three enzymes compared to values for 4FP. However, the Km value for 3,4FP decreased for P. putida F6 tyr indicating a higher affinity for P. putida F6 tyr for 3,4FP compared to 4FP. Furthermore the k(cat) value for 3,4FP increased for mushroom tyr in comparison to the value for 4FP indicating a higher maximum turnover of 3,4FP compared to 4FP for mushroom tyr. All three sources of tyr exhibited lower catalytic efficiencies for 3-fluorophenol (3FP) and 2-fluorophenol (2FP) compared to 4FP. However, the K-m value for 2FP was lower than that for 3FP for both S. antibioticus and mushroom tyr indicating a higher affinity for 2FP over 3FP. (C) 2008 Elsevier Inc. All rights reserved. | en |
dc.publisher | Elsevier Science Inc, New York | |
dc.rights | restrictedAccess | |
dc.source | Enzyme and Microbial Technology | |
dc.subject | Pseudomonas putida F6 | en |
dc.subject | Streptomyces antibioticus | en |
dc.subject | Agaricus bisporus (mushroom) | en |
dc.subject | tyrosinase | en |
dc.subject | 4-fluorophenol | en |
dc.subject | 3,4-difluorophenol | en |
dc.subject | 3-fluorophenol | en |
dc.subject | 2-fluorophenol | en |
dc.title | Assessing the catalytic activity of three different sources of tyrosinase: A study of the oxidation of mono- and difluorinated monophenols | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Мартин, Леона Б.; Вијгенбоом, Ерик; Никодиновић-Рунић, Јасмина; МцМахон, Aоифе М.; О'Цоннор, Кевин Е.; | |
dc.citation.volume | 43 | |
dc.citation.issue | 3 | |
dc.citation.spage | 297 | |
dc.citation.epage | 301 | |
dc.identifier.wos | 000258217800011 | |
dc.identifier.doi | 10.1016/j.enzmictec.2008.03.010 | |
dc.citation.other | 43(3): 297-301 | |
dc.citation.rank | M22 | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-46749111362 |
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