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Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)

dc.creatorBožić, Nataša
dc.creatorDojnov, Biljana
dc.creatorMilovanovic, Aleksandra
dc.creatorNenadovic, Vera
dc.creatorIvanovic, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2018-11-22T00:13:28Z
dc.date.available2018-11-22T00:13:28Z
dc.date.issued2008
dc.identifier.issn0354-4664
dc.identifier.urihttps://cherry.chem.bg.ac.rs/handle/123456789/963
dc.description.abstractApplication of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.en
dc.description.abstractPrimenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.sr
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovic, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceArchives of biological sciences
dc.subjectcerambycid beetleen
dc.subjectMorimus funereusen
dc.subjectMorimus funereusen
dc.subjectmidgut peptidasesen
dc.subjectmidgut peptidasesen
dc.subjectprotease inhibitorsen
dc.subjectprotease inhibitorsen
dc.subjectsynthetic substrateen
dc.subjectsynthetic substrateen
dc.subjectzymogramen
dc.subjectzymogramen
dc.titleCharacterization of endopeptidases from the midgut of Morimus funereus (Coleoptera : Cerambycidae) larvaeen
dc.titleKarakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractБозиц, Натаса; Вујчић, Зоран; Ивановиц, Јелисавета; Ненадовиц, Вера; Миловановиц, Aлександра; Дојнов, Биљана;
dc.citation.volume60
dc.citation.issue3
dc.citation.spage403
dc.citation.epage409
dc.identifier.wos000258524700008
dc.identifier.doi10.2298/ABS0803403B
dc.citation.other60(3): 403-409
dc.type.versionpublishedVersion
dc.identifier.scopus2-s2.0-53549104726
dc.identifier.fulltexthttps://cherry.chem.bg.ac.rs/bitstream/id/10134/961.pdf


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