Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method
Samo za registrovane korisnike
2008
Autori
Vidovic, StojankaStojanovic, Bijana
Veljkovic, Jelena
Prazic-Arsic, Ljiljana
Roglić, Goran
Manojlović, Dragan D.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
HPLC stability-indicating method has been developed for the simultaneous determination of some water-soluble vitamins (ascorbic acid, thiamine hydrochloride, riboflavin-5'-phosphate sodium, pyridoxine hydrochloride, nicotinamide, D(+)-panthenol) and two preservatives (methylparaben and sodium benzoate) in multivitamin syrup preparation. Water-soluble vitamins, preservatives and their degradants were separated on Zorbax SB-Aq (C-18) (250 mm x 4.6 mm, 5 mu m) column at an ambient temperature. Combined isocratic and gradient elution was performed with a mobile phase consisting of 0.0125M hexane-1-sulfonic acid sodium salt in 0.1% (m/v) o-phosphoric acid, pH 2.4-2.5 (solvent A) and acetonitrile (solvent B) at the flow-rate 1 ml min(-1). Starting with solvent A an isocratic elution was performed for 15 min, then the composition was changed to 85% of A and 15% of B during the next 20 min and it was constant for 5 min, then the composition was changed to 70% of A and 30% of B during next 15 m...in and it was constant for 5 min and finally was changed to 100% of A as at the beginning of the elution. Detection was performed with diode array detector at 210, 230 and 254 nm. Multivitamin syrup preparation was subjected to stress testing (forced degradation) in order to demonstrate that degradants from the vitamins, preservatives and/or product excipients do not interfere with the quantification of vitamins and preservatives. Typical validation characteristics: selectivity, accuracy, precision, linearity, range, limit of quantification and limit of detection were evaluated for vitamins and preservatives. (C) 2008 Elsevier B.V. All rights reserved.
Ključne reči:
water-soluble vitamins / preservatives / HPLC stability-indicating / stress testing / multivitamin syrupIzvor:
Journal of Chromatography A, 2008, 1202, 2, 155-162Izdavač:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/j.chroma.2008.06.039
ISSN: 0021-9673
PubMed: 18644604
WoS: 000258696800007
Scopus: 2-s2.0-48349134530
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vidovic, Stojanka AU - Stojanovic, Bijana AU - Veljkovic, Jelena AU - Prazic-Arsic, Ljiljana AU - Roglić, Goran AU - Manojlović, Dragan D. PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/965 AB - HPLC stability-indicating method has been developed for the simultaneous determination of some water-soluble vitamins (ascorbic acid, thiamine hydrochloride, riboflavin-5'-phosphate sodium, pyridoxine hydrochloride, nicotinamide, D(+)-panthenol) and two preservatives (methylparaben and sodium benzoate) in multivitamin syrup preparation. Water-soluble vitamins, preservatives and their degradants were separated on Zorbax SB-Aq (C-18) (250 mm x 4.6 mm, 5 mu m) column at an ambient temperature. Combined isocratic and gradient elution was performed with a mobile phase consisting of 0.0125M hexane-1-sulfonic acid sodium salt in 0.1% (m/v) o-phosphoric acid, pH 2.4-2.5 (solvent A) and acetonitrile (solvent B) at the flow-rate 1 ml min(-1). Starting with solvent A an isocratic elution was performed for 15 min, then the composition was changed to 85% of A and 15% of B during the next 20 min and it was constant for 5 min, then the composition was changed to 70% of A and 30% of B during next 15 min and it was constant for 5 min and finally was changed to 100% of A as at the beginning of the elution. Detection was performed with diode array detector at 210, 230 and 254 nm. Multivitamin syrup preparation was subjected to stress testing (forced degradation) in order to demonstrate that degradants from the vitamins, preservatives and/or product excipients do not interfere with the quantification of vitamins and preservatives. Typical validation characteristics: selectivity, accuracy, precision, linearity, range, limit of quantification and limit of detection were evaluated for vitamins and preservatives. (C) 2008 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Chromatography A T1 - Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method VL - 1202 IS - 2 SP - 155 EP - 162 DO - 10.1016/j.chroma.2008.06.039 ER -
@article{ author = "Vidovic, Stojanka and Stojanovic, Bijana and Veljkovic, Jelena and Prazic-Arsic, Ljiljana and Roglić, Goran and Manojlović, Dragan D.", year = "2008", abstract = "HPLC stability-indicating method has been developed for the simultaneous determination of some water-soluble vitamins (ascorbic acid, thiamine hydrochloride, riboflavin-5'-phosphate sodium, pyridoxine hydrochloride, nicotinamide, D(+)-panthenol) and two preservatives (methylparaben and sodium benzoate) in multivitamin syrup preparation. Water-soluble vitamins, preservatives and their degradants were separated on Zorbax SB-Aq (C-18) (250 mm x 4.6 mm, 5 mu m) column at an ambient temperature. Combined isocratic and gradient elution was performed with a mobile phase consisting of 0.0125M hexane-1-sulfonic acid sodium salt in 0.1% (m/v) o-phosphoric acid, pH 2.4-2.5 (solvent A) and acetonitrile (solvent B) at the flow-rate 1 ml min(-1). Starting with solvent A an isocratic elution was performed for 15 min, then the composition was changed to 85% of A and 15% of B during the next 20 min and it was constant for 5 min, then the composition was changed to 70% of A and 30% of B during next 15 min and it was constant for 5 min and finally was changed to 100% of A as at the beginning of the elution. Detection was performed with diode array detector at 210, 230 and 254 nm. Multivitamin syrup preparation was subjected to stress testing (forced degradation) in order to demonstrate that degradants from the vitamins, preservatives and/or product excipients do not interfere with the quantification of vitamins and preservatives. Typical validation characteristics: selectivity, accuracy, precision, linearity, range, limit of quantification and limit of detection were evaluated for vitamins and preservatives. (C) 2008 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Chromatography A", title = "Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method", volume = "1202", number = "2", pages = "155-162", doi = "10.1016/j.chroma.2008.06.039" }
Vidovic, S., Stojanovic, B., Veljkovic, J., Prazic-Arsic, L., Roglić, G.,& Manojlović, D. D.. (2008). Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method. in Journal of Chromatography A Elsevier Science Bv, Amsterdam., 1202(2), 155-162. https://doi.org/10.1016/j.chroma.2008.06.039
Vidovic S, Stojanovic B, Veljkovic J, Prazic-Arsic L, Roglić G, Manojlović DD. Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method. in Journal of Chromatography A. 2008;1202(2):155-162. doi:10.1016/j.chroma.2008.06.039 .
Vidovic, Stojanka, Stojanovic, Bijana, Veljkovic, Jelena, Prazic-Arsic, Ljiljana, Roglić, Goran, Manojlović, Dragan D., "Simultaneous determination of some water-soluble vitamins and preservatives in multivitamin syrup by validated stability-indicating high-performance liquid chromatography method" in Journal of Chromatography A, 1202, no. 2 (2008):155-162, https://doi.org/10.1016/j.chroma.2008.06.039 . .