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dc.creatorNikodinović-Runić, Jasmina
dc.creatorKenny, Shane T.
dc.creatorBabu, Ramesh P.
dc.creatorWoods, Trevor
dc.creatorBlau, Werner J.
dc.creatorO'Connor, Kevin E.
dc.date.accessioned2018-11-22T00:13:32Z
dc.date.available2018-11-22T00:13:32Z
dc.date.issued2008
dc.identifier.issn0175-7598
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/967
dc.description.abstractHere, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.en
dc.publisherSpringer, New York
dc.rightsrestrictedAccess
dc.sourceApplied Microbiology and Biotechnology
dc.titleThe conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoateen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКеннy, Схане Т.; Никодиновић-Рунић, Јасмина; Блау, Wернер Ј.; О'Цоннор, Кевин Е.; Бабу, Рамесх П.; Wоодс, Тревор;
dc.citation.volume80
dc.citation.issue4
dc.citation.spage665
dc.citation.epage673
dc.identifier.wos000258881400013
dc.identifier.doi10.1007/s00253-008-1593-0
dc.citation.other80(4): 665-673
dc.citation.rankM22
dc.identifier.pmid18629491
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-50849084452
dc.identifier.rcubKon_1920


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