Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae
Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
Abstract
The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.
pH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom.
Keywords:
Cerambycidae / Morimus funereus / Morimus funereus / trypsin / trypsin / midgut peptidases / midgut peptidases / synthetic substrate / synthetic substrate / zymogram / zymogramSource:
Archives of biological sciences, 2009, 61, 4, 713-718Publisher:
- Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
Funding / projects:
- Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama (RS-MESTD-MPN2006-2010-142026)
DOI: 10.2298/ABS0904713L
ISSN: 0354-4664
WoS: 000273203500018
Scopus: 2-s2.0-77949879656
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Lončar, Nikola L. AU - Božić, Nataša AU - Nenadovic, Vera AU - Ivanovic, Jelisaveta AU - Vujčić, Zoran PY - 2009 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1038 AB - The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine. AB - pH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom. PB - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd T2 - Archives of biological sciences T1 - Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae T1 - Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae) VL - 61 IS - 4 SP - 713 EP - 718 DO - 10.2298/ABS0904713L ER -
@article{ author = "Lončar, Nikola L. and Božić, Nataša and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran", year = "2009", abstract = "The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine., pH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom.", publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd", journal = "Archives of biological sciences", title = "Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae, Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)", volume = "61", number = "4", pages = "713-718", doi = "10.2298/ABS0904713L" }
Lončar, N. L., Božić, N., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2009). Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 61(4), 713-718. https://doi.org/10.2298/ABS0904713L
Lončar NL, Božić N, Nenadovic V, Ivanovic J, Vujčić Z. Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences. 2009;61(4):713-718. doi:10.2298/ABS0904713L .
Lončar, Nikola L., Božić, Nataša, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Characterization of Trypsin-Like Enzymes from the Midgut of Morimus Funereus (Coleoptera: Cerambycidae) Larvae" in Archives of biological sciences, 61, no. 4 (2009):713-718, https://doi.org/10.2298/ABS0904713L . .