Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography
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Tosti, TomislavNatić, Maja
Smolinski, A.
Milić, Dragana
Milojković-Opsenica, Dušanka
Tešić, Živoslav Lj.
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The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1: 9 (v/v). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal-and reversed-phase systems used, retention paramet...ers were investigated by principal component analysis and cluster analysis.
Keywords:
estrone-derived synthetic steroids / principal component analysis / hierarchical cluster analysisSource:
Acta Chromatographica, 2011, 23, 3, 429-445Publisher:
- Akademiai Kiado Rt, Budapest
Funding / projects:
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
DOI: 10.1556/AChrom.23.2011.3.5
ISSN: 1233-2356
WoS: 000294569400005
Scopus: 2-s2.0-80052659404
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Tosti, Tomislav AU - Natić, Maja AU - Smolinski, A. AU - Milić, Dragana AU - Milojković-Opsenica, Dušanka AU - Tešić, Živoslav Lj. PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1196 AB - The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1: 9 (v/v). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal-and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis. PB - Akademiai Kiado Rt, Budapest T2 - Acta Chromatographica T1 - Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography VL - 23 IS - 3 SP - 429 EP - 445 DO - 10.1556/AChrom.23.2011.3.5 ER -
@article{ author = "Tosti, Tomislav and Natić, Maja and Smolinski, A. and Milić, Dragana and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj.", year = "2011", abstract = "The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1: 9 (v/v). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal-and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis.", publisher = "Akademiai Kiado Rt, Budapest", journal = "Acta Chromatographica", title = "Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography", volume = "23", number = "3", pages = "429-445", doi = "10.1556/AChrom.23.2011.3.5" }
Tosti, T., Natić, M., Smolinski, A., Milić, D., Milojković-Opsenica, D.,& Tešić, Ž. Lj.. (2011). Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography. in Acta Chromatographica Akademiai Kiado Rt, Budapest., 23(3), 429-445. https://doi.org/10.1556/AChrom.23.2011.3.5
Tosti T, Natić M, Smolinski A, Milić D, Milojković-Opsenica D, Tešić ŽL. Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography. in Acta Chromatographica. 2011;23(3):429-445. doi:10.1556/AChrom.23.2011.3.5 .
Tosti, Tomislav, Natić, Maja, Smolinski, A., Milić, Dragana, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., "Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography" in Acta Chromatographica, 23, no. 3 (2011):429-445, https://doi.org/10.1556/AChrom.23.2011.3.5 . .