Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase
Apstrakt
One of the key elements for understanding enzyme reactions is determination of its kinetic parameters. Since transglucosylation is kinetically controlled reaction, besides the reaction of synthesis, very important is the reaction of enzymatic hydrolysis of created product. Therefore, in this study, kinetic parameters for synthesis and secondary hydrolysis of pharmacologically active alpha isosalicin by baker's yeast maltase were calculated, and it was shown that specifity of maltase for hydrolysis is approximately 150 times higher then for synthesis.
Ključne reči:
synthesis and hydrolysis of salicin isomer / catalysis by maltase / kinetic parametersIzvor:
Russian Journal of Physical Chemistry A, 2011, 85, 13, 2317-2321Izdavač:
- Maik Nauka/Interperiodica/Springer, New York
Finansiranje / projekti:
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1134/S0036024411130346
ISSN: 0036-0244
WoS: 000297922700014
Scopus: 2-s2.0-84872396003
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Velickovic, D. V. AU - Dimitrijevic, A. S. AU - Bihelović, Filip AU - Jankov, Ratko M. AU - Milosavic, N. PY - 2011 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1231 AB - One of the key elements for understanding enzyme reactions is determination of its kinetic parameters. Since transglucosylation is kinetically controlled reaction, besides the reaction of synthesis, very important is the reaction of enzymatic hydrolysis of created product. Therefore, in this study, kinetic parameters for synthesis and secondary hydrolysis of pharmacologically active alpha isosalicin by baker's yeast maltase were calculated, and it was shown that specifity of maltase for hydrolysis is approximately 150 times higher then for synthesis. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Russian Journal of Physical Chemistry A T1 - Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase VL - 85 IS - 13 SP - 2317 EP - 2321 DO - 10.1134/S0036024411130346 ER -
@article{ author = "Velickovic, D. V. and Dimitrijevic, A. S. and Bihelović, Filip and Jankov, Ratko M. and Milosavic, N.", year = "2011", abstract = "One of the key elements for understanding enzyme reactions is determination of its kinetic parameters. Since transglucosylation is kinetically controlled reaction, besides the reaction of synthesis, very important is the reaction of enzymatic hydrolysis of created product. Therefore, in this study, kinetic parameters for synthesis and secondary hydrolysis of pharmacologically active alpha isosalicin by baker's yeast maltase were calculated, and it was shown that specifity of maltase for hydrolysis is approximately 150 times higher then for synthesis.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Russian Journal of Physical Chemistry A", title = "Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase", volume = "85", number = "13", pages = "2317-2321", doi = "10.1134/S0036024411130346" }
Velickovic, D. V., Dimitrijevic, A. S., Bihelović, F., Jankov, R. M.,& Milosavic, N.. (2011). Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase. in Russian Journal of Physical Chemistry A Maik Nauka/Interperiodica/Springer, New York., 85(13), 2317-2321. https://doi.org/10.1134/S0036024411130346
Velickovic DV, Dimitrijevic AS, Bihelović F, Jankov RM, Milosavic N. Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase. in Russian Journal of Physical Chemistry A. 2011;85(13):2317-2321. doi:10.1134/S0036024411130346 .
Velickovic, D. V., Dimitrijevic, A. S., Bihelović, Filip, Jankov, Ratko M., Milosavic, N., "Study of the kinetic parameters for synthesis and hydrolysis of pharmacologically active salicin isomer catalyzed by baker's yeast maltase" in Russian Journal of Physical Chemistry A, 85, no. 13 (2011):2317-2321, https://doi.org/10.1134/S0036024411130346 . .