Recombinant banana lectin as mucosal immunostimulator
Abstract
To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. (c) 2012 Elsevier Ltd. All rights reserved.
Keywords:
Recombinant banana lectin / Mouse digestive tract / Antibody subclass profileSource:
Journal of Functional Foods, 2012, 4, 3, 636-641Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
- The pathogenetic mechanism in hematological malignancies (RS-MESTD-Basic Research (BR or ON)-175053)
DOI: 10.1016/j.jff.2012.04.003
ISSN: 1756-4646
WoS: 000305492100008
Scopus: 2-s2.0-84861671490
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Dimitrijevic, R. AU - Stojanovic, M. AU - Micic, M. AU - Dimitrijevic, Lj AU - Gavrović-Jankulović, Marija PY - 2012 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1310 AB - To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. (c) 2012 Elsevier Ltd. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Functional Foods T1 - Recombinant banana lectin as mucosal immunostimulator VL - 4 IS - 3 SP - 636 EP - 641 DO - 10.1016/j.jff.2012.04.003 ER -
@article{ author = "Dimitrijevic, R. and Stojanovic, M. and Micic, M. and Dimitrijevic, Lj and Gavrović-Jankulović, Marija", year = "2012", abstract = "To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. (c) 2012 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Functional Foods", title = "Recombinant banana lectin as mucosal immunostimulator", volume = "4", number = "3", pages = "636-641", doi = "10.1016/j.jff.2012.04.003" }
Dimitrijevic, R., Stojanovic, M., Micic, M., Dimitrijevic, L.,& Gavrović-Jankulović, M.. (2012). Recombinant banana lectin as mucosal immunostimulator. in Journal of Functional Foods Elsevier Science Bv, Amsterdam., 4(3), 636-641. https://doi.org/10.1016/j.jff.2012.04.003
Dimitrijevic R, Stojanovic M, Micic M, Dimitrijevic L, Gavrović-Jankulović M. Recombinant banana lectin as mucosal immunostimulator. in Journal of Functional Foods. 2012;4(3):636-641. doi:10.1016/j.jff.2012.04.003 .
Dimitrijevic, R., Stojanovic, M., Micic, M., Dimitrijevic, Lj, Gavrović-Jankulović, Marija, "Recombinant banana lectin as mucosal immunostimulator" in Journal of Functional Foods, 4, no. 3 (2012):636-641, https://doi.org/10.1016/j.jff.2012.04.003 . .