Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA
Само за регистроване кориснике
2013
Аутори
Vujčić, MiroslavaTufegdžić, Srđan
Novaković, Irena T.
Djikanovic, Daniela
Gasic, Miroslav J.
Sladić, Dušan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by t...he observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA.
Кључне речи:
Avarol / Avarone / 3 '-(Methylamino)avarone / 4 '-(Methylamino)avarone / Calf thymus DNA / Fluorescence measurementsИзвор:
International Journal of Biological Macromolecules, 2013, 62, 405-410Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Интеракције природних производа, њихових деривата и комплексних једињења са протеинима и нуклеинским киселинама (RS-MESTD-Basic Research (BR or ON)-172055)
DOI: 10.1016/j.ijbiomac.2013.09.013
ISSN: 0141-8130
PubMed: 24080453
WoS: 000329881200060
Scopus: 2-s2.0-84886041638
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vujčić, Miroslava AU - Tufegdžić, Srđan AU - Novaković, Irena T. AU - Djikanovic, Daniela AU - Gasic, Miroslav J. AU - Sladić, Dušan PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1484 AB - The interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by the observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA. PB - Elsevier Science Bv, Amsterdam T2 - International Journal of Biological Macromolecules T1 - Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA VL - 62 SP - 405 EP - 410 DO - 10.1016/j.ijbiomac.2013.09.013 ER -
@article{ author = "Vujčić, Miroslava and Tufegdžić, Srđan and Novaković, Irena T. and Djikanovic, Daniela and Gasic, Miroslav J. and Sladić, Dušan", year = "2013", abstract = "The interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by the observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Journal of Biological Macromolecules", title = "Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA", volume = "62", pages = "405-410", doi = "10.1016/j.ijbiomac.2013.09.013" }
Vujčić, M., Tufegdžić, S., Novaković, I. T., Djikanovic, D., Gasic, M. J.,& Sladić, D.. (2013). Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA. in International Journal of Biological Macromolecules Elsevier Science Bv, Amsterdam., 62, 405-410. https://doi.org/10.1016/j.ijbiomac.2013.09.013
Vujčić M, Tufegdžić S, Novaković IT, Djikanovic D, Gasic MJ, Sladić D. Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA. in International Journal of Biological Macromolecules. 2013;62:405-410. doi:10.1016/j.ijbiomac.2013.09.013 .
Vujčić, Miroslava, Tufegdžić, Srđan, Novaković, Irena T., Djikanovic, Daniela, Gasic, Miroslav J., Sladić, Dušan, "Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA" in International Journal of Biological Macromolecules, 62 (2013):405-410, https://doi.org/10.1016/j.ijbiomac.2013.09.013 . .