The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate
Samo za registrovane korisnike
2013
Autori
O'Connor, StephenSzwej, Emilia
Nikodinović-Runić, Jasmina
O'Connor, Aisling
Byrne, Annette T.
Devocelle, Marc
O'Donovan, Norma
Gallagher, William M.
Babu, Ramesh P.
Kenny, Shane T.
Zinn, Manfred
Zulian, Qun Ren
O'Connor, Kevin E.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values c...ompared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.
Ključne reči:
Polyhydroxyalkanoate / Monomer / (R)-3-hydroxydecanoic acid / Antimicrobial peptides / Apoptosis / Cancer biologyIzvor:
Biomaterials, 2013, 34, 11, 2710-2718Izdavač:
- Elsevier Sci Ltd, Oxford
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3550
DOI: 10.1016/j.biomaterials.2012.12.032
ISSN: 0142-9612
PubMed: 23343631
WoS: 000315748200014
Scopus: 2-s2.0-84873085844
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - O'Connor, Stephen AU - Szwej, Emilia AU - Nikodinović-Runić, Jasmina AU - O'Connor, Aisling AU - Byrne, Annette T. AU - Devocelle, Marc AU - O'Donovan, Norma AU - Gallagher, William M. AU - Babu, Ramesh P. AU - Kenny, Shane T. AU - Zinn, Manfred AU - Zulian, Qun Ren AU - O'Connor, Kevin E. PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1609 AB - The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range. PB - Elsevier Sci Ltd, Oxford T2 - Biomaterials T1 - The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate VL - 34 IS - 11 SP - 2710 EP - 2718 DO - 10.1016/j.biomaterials.2012.12.032 ER -
@article{ author = "O'Connor, Stephen and Szwej, Emilia and Nikodinović-Runić, Jasmina and O'Connor, Aisling and Byrne, Annette T. and Devocelle, Marc and O'Donovan, Norma and Gallagher, William M. and Babu, Ramesh P. and Kenny, Shane T. and Zinn, Manfred and Zulian, Qun Ren and O'Connor, Kevin E.", year = "2013", abstract = "The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Biomaterials", title = "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate", volume = "34", number = "11", pages = "2710-2718", doi = "10.1016/j.biomaterials.2012.12.032" }
O'Connor, S., Szwej, E., Nikodinović-Runić, J., O'Connor, A., Byrne, A. T., Devocelle, M., O'Donovan, N., Gallagher, W. M., Babu, R. P., Kenny, S. T., Zinn, M., Zulian, Q. R.,& O'Connor, K. E.. (2013). The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials Elsevier Sci Ltd, Oxford., 34(11), 2710-2718. https://doi.org/10.1016/j.biomaterials.2012.12.032
O'Connor S, Szwej E, Nikodinović-Runić J, O'Connor A, Byrne AT, Devocelle M, O'Donovan N, Gallagher WM, Babu RP, Kenny ST, Zinn M, Zulian QR, O'Connor KE. The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials. 2013;34(11):2710-2718. doi:10.1016/j.biomaterials.2012.12.032 .
O'Connor, Stephen, Szwej, Emilia, Nikodinović-Runić, Jasmina, O'Connor, Aisling, Byrne, Annette T., Devocelle, Marc, O'Donovan, Norma, Gallagher, William M., Babu, Ramesh P., Kenny, Shane T., Zinn, Manfred, Zulian, Qun Ren, O'Connor, Kevin E., "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate" in Biomaterials, 34, no. 11 (2013):2710-2718, https://doi.org/10.1016/j.biomaterials.2012.12.032 . .