Dog saliva - an important source of dog allergens
2013
Authors
Polović, NatalijaWaden, Konrad
Binnmyr, J.
Hamsten, Carl
Gronneberg, R.
Palmberg, C.
Milčić-Matić, Natalija
Bergman, T.
Gronlund, Hans
van Hage, Marianne
Article (Published version)
Metadata
Show full item recordAbstract
Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander ...extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
Keywords:
allergen / Canis familiaris / diagnosis / dog allergy / salivaSource:
Allergy, 2013, 68, 5, 585-592Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
- Swedish Research Council
- Stockholm County Council
- Hesselman Foundation
- Swedish Asthma and Allergy Associations Research Foundation
- Swedish Heart-Lung Foundation
- King Gustaf V 80th Birthday Foundation
- Karolinska Institutet
- Bernard Osher Initiative for Research on Severe Asthma at Karolinska Institutet
- Swedish Cancer and Allergy Foundation
- Center for Inflammatory Diseases
- Centre for Allergy Research
- Konsul Th C Bergh Foundation
- EAACI
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3488
DOI: 10.1111/all.12130
ISSN: 0105-4538
PubMed: 23464525
WoS: 000317981500006
Scopus: 2-s2.0-84876688875
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Polović, Natalija AU - Waden, Konrad AU - Binnmyr, J. AU - Hamsten, Carl AU - Gronneberg, R. AU - Palmberg, C. AU - Milčić-Matić, Natalija AU - Bergman, T. AU - Gronlund, Hans AU - van Hage, Marianne PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1634 AB - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies. PB - Wiley-Blackwell, Hoboken T2 - Allergy T1 - Dog saliva - an important source of dog allergens VL - 68 IS - 5 SP - 585 EP - 592 DO - 10.1111/all.12130 ER -
@article{ author = "Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne", year = "2013", abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.", publisher = "Wiley-Blackwell, Hoboken", journal = "Allergy", title = "Dog saliva - an important source of dog allergens", volume = "68", number = "5", pages = "585-592", doi = "10.1111/all.12130" }
Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy Wiley-Blackwell, Hoboken., 68(5), 585-592. https://doi.org/10.1111/all.12130
Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy. 2013;68(5):585-592. doi:10.1111/all.12130 .
Polović, Natalija, Waden, Konrad, Binnmyr, J., Hamsten, Carl, Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, Hans, van Hage, Marianne, "Dog saliva - an important source of dog allergens" in Allergy, 68, no. 5 (2013):585-592, https://doi.org/10.1111/all.12130 . .