Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization
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2015
Authors
Vujčić, ZoranJanović, Barbara
Lončar, Nikola L.
Margetić, Aleksandra
Božić, Nataša
Dojnov, Biljana
Vujčić, Miroslava
Article (Published version)
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Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but... leaves chromophore present. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords:
Horseradish peroxidase / HRP / Decolorization / Reactive dyes / Zymogram / IzoenzymesSource:
International Biodeterioration and Biodegradation, 2015, 97, 124-127Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
DOI: 10.1016/j.ibiod.2014.10.007
ISSN: 0964-8305
WoS: 000348557700016
Scopus: 2-s2.0-84912567704
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Vujčić, Zoran AU - Janović, Barbara AU - Lončar, Nikola L. AU - Margetić, Aleksandra AU - Božić, Nataša AU - Dojnov, Biljana AU - Vujčić, Miroslava PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1644 AB - Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present. (C) 2014 Elsevier Ltd. All rights reserved. PB - Elsevier Sci Ltd, Oxford T2 - International Biodeterioration and Biodegradation T1 - Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization VL - 97 SP - 124 EP - 127 DO - 10.1016/j.ibiod.2014.10.007 ER -
@article{ author = "Vujčić, Zoran and Janović, Barbara and Lončar, Nikola L. and Margetić, Aleksandra and Božić, Nataša and Dojnov, Biljana and Vujčić, Miroslava", year = "2015", abstract = "Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present. (C) 2014 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Sci Ltd, Oxford", journal = "International Biodeterioration and Biodegradation", title = "Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization", volume = "97", pages = "124-127", doi = "10.1016/j.ibiod.2014.10.007" }
Vujčić, Z., Janović, B., Lončar, N. L., Margetić, A., Božić, N., Dojnov, B.,& Vujčić, M.. (2015). Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization. in International Biodeterioration and Biodegradation Elsevier Sci Ltd, Oxford., 97, 124-127. https://doi.org/10.1016/j.ibiod.2014.10.007
Vujčić Z, Janović B, Lončar NL, Margetić A, Božić N, Dojnov B, Vujčić M. Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization. in International Biodeterioration and Biodegradation. 2015;97:124-127. doi:10.1016/j.ibiod.2014.10.007 .
Vujčić, Zoran, Janović, Barbara, Lončar, Nikola L., Margetić, Aleksandra, Božić, Nataša, Dojnov, Biljana, Vujčić, Miroslava, "Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization" in International Biodeterioration and Biodegradation, 97 (2015):124-127, https://doi.org/10.1016/j.ibiod.2014.10.007 . .