Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples
Abstract
This paper presents, for the first time, electrochemical behavior of diterpene lactone andrographolide and its voltammetric determination using differential pulse voltammetry (DPV) at boron-doped diamond electrode, in human urine and oil extract from Andrographis paniculata seeds. Andrographolide, due to its chemical structure, provides well defined peak at +1.55 V vs. Ag/AgCl electrode (3 M) in Britton-Robinson buffer solution pH 12. The proposed method, under optimum experimental parameters displays excellent selectivity towards andrographolide in the presence of some interference, as ascorbic acid, uric acid and dopamine, with detection limit of 0.75 mu M. Calibration curves were found to be linear in the range from 2 to 40 mu M, with two linear ranges, first from 2 to 10 mu M and second from 10 to 40 mu M with corresponding equations I (mu A) = (4.00 +/- 0.12) c (mu M) + (13.42 +/- 0.66) (R-2 = 0.9975) and I (mu A) = (0.87 +/- 0.05) c (mu M) + (46.33 +/- 1.53) (R-2 = 0.9767), respe...ctively. Relative standard deviation of 7 measurements was 4% which verified excellent repeatability of proposed method and this method was successfully applied for the determination of andrographolide in human urine sample and extract from the A. paniculata seeds. The Prussian blue method was used for validation of the proposed elactroanalytical method. (C) 2015 Elsevier B.V. All rights reserved.
Keywords:
Andrographolide / Andrographis paniculata / Human urine / Boron-doped diamond electrodeSource:
Microchemical Journal, 2015, 122, 16-19Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
DOI: 10.1016/j.microc.2015.04.005
ISSN: 0026-265X
WoS: 000356748200004
Scopus: 2-s2.0-84927632277
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Stanković, Dalibor AU - Samphao, Anchalee AU - Kuzmanović, Darko AU - Kalcher, Kurt PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1725 AB - This paper presents, for the first time, electrochemical behavior of diterpene lactone andrographolide and its voltammetric determination using differential pulse voltammetry (DPV) at boron-doped diamond electrode, in human urine and oil extract from Andrographis paniculata seeds. Andrographolide, due to its chemical structure, provides well defined peak at +1.55 V vs. Ag/AgCl electrode (3 M) in Britton-Robinson buffer solution pH 12. The proposed method, under optimum experimental parameters displays excellent selectivity towards andrographolide in the presence of some interference, as ascorbic acid, uric acid and dopamine, with detection limit of 0.75 mu M. Calibration curves were found to be linear in the range from 2 to 40 mu M, with two linear ranges, first from 2 to 10 mu M and second from 10 to 40 mu M with corresponding equations I (mu A) = (4.00 +/- 0.12) c (mu M) + (13.42 +/- 0.66) (R-2 = 0.9975) and I (mu A) = (0.87 +/- 0.05) c (mu M) + (46.33 +/- 1.53) (R-2 = 0.9767), respectively. Relative standard deviation of 7 measurements was 4% which verified excellent repeatability of proposed method and this method was successfully applied for the determination of andrographolide in human urine sample and extract from the A. paniculata seeds. The Prussian blue method was used for validation of the proposed elactroanalytical method. (C) 2015 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Microchemical Journal T1 - Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples VL - 122 SP - 16 EP - 19 DO - 10.1016/j.microc.2015.04.005 ER -
@article{ author = "Stanković, Dalibor and Samphao, Anchalee and Kuzmanović, Darko and Kalcher, Kurt", year = "2015", abstract = "This paper presents, for the first time, electrochemical behavior of diterpene lactone andrographolide and its voltammetric determination using differential pulse voltammetry (DPV) at boron-doped diamond electrode, in human urine and oil extract from Andrographis paniculata seeds. Andrographolide, due to its chemical structure, provides well defined peak at +1.55 V vs. Ag/AgCl electrode (3 M) in Britton-Robinson buffer solution pH 12. The proposed method, under optimum experimental parameters displays excellent selectivity towards andrographolide in the presence of some interference, as ascorbic acid, uric acid and dopamine, with detection limit of 0.75 mu M. Calibration curves were found to be linear in the range from 2 to 40 mu M, with two linear ranges, first from 2 to 10 mu M and second from 10 to 40 mu M with corresponding equations I (mu A) = (4.00 +/- 0.12) c (mu M) + (13.42 +/- 0.66) (R-2 = 0.9975) and I (mu A) = (0.87 +/- 0.05) c (mu M) + (46.33 +/- 1.53) (R-2 = 0.9767), respectively. Relative standard deviation of 7 measurements was 4% which verified excellent repeatability of proposed method and this method was successfully applied for the determination of andrographolide in human urine sample and extract from the A. paniculata seeds. The Prussian blue method was used for validation of the proposed elactroanalytical method. (C) 2015 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Microchemical Journal", title = "Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples", volume = "122", pages = "16-19", doi = "10.1016/j.microc.2015.04.005" }
Stanković, D., Samphao, A., Kuzmanović, D.,& Kalcher, K.. (2015). Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples. in Microchemical Journal Elsevier Science Bv, Amsterdam., 122, 16-19. https://doi.org/10.1016/j.microc.2015.04.005
Stanković D, Samphao A, Kuzmanović D, Kalcher K. Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples. in Microchemical Journal. 2015;122:16-19. doi:10.1016/j.microc.2015.04.005 .
Stanković, Dalibor, Samphao, Anchalee, Kuzmanović, Darko, Kalcher, Kurt, "Novel electroanalyical method for the determination of andrographolide from Andrographis paniculata extract and urine samples" in Microchemical Journal, 122 (2015):16-19, https://doi.org/10.1016/j.microc.2015.04.005 . .