Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode
Apstrakt
For the first time, a simple and sensitive analytical method for the direct determination of yohimbine is presented using differential pulse voltammetry with a boron-doped diamond electrode. Two irreversible oxidation peaks, a distinct one at +0.80 and a second poorly-defined one at +1.65 V, were observed when cyclic voltammetry was carried out in Britton-Robinson buffer solution at pH 7 (vs. Ag/AgCl). With optimized differential pulse voltammetric parameters (pulse amplitude 100 mV, pulse time 25 ms, step potential 5 mV and scan rate 10 mV s(-1)), the current response of yohimbine at +0.80 V was linearly proportional to the concentration in the range from 0.25 to 90.9 mu mol L-1 with a low detection limit of 0.13 mu mol L-1 (0.046 mg L-1) and a good repeatability (relative standard deviation of 2.5% at 18.4 mu mol L-1 for n = 6). The practical applicability of the developed method was demonstrated by the assessment of the total content of yohimbine in extracts of the primary bark of n...atural aphrodisiacs such as Pausinystalia yohimbe and Rauvolfia serpentina with recoveries in the range of 92-97%. The proposed electrochemical procedure represents an inexpensive and effective analytical alternative for the quality control analysis of products containing yohimbine and other biologically and structurally related alkaloids used as natural dietary supplements.
Izvor:
Analytical Methods, 2014, 6, 13, 4853-4859Izdavač:
- Royal Soc Chemistry, Cambridge
Finansiranje / projekti:
- Primena unapređenih oksidacionih procesa i nanostrukturisanih oksidnih materijala za uklanjanje zagađivača iz životne sredine, razvoj i optimizacija instrumentalnih tehnika za praćenje efikasnosti (RS-172030)
- Bilateral program Action: Austria-Slovakia
- Slovak Research and Development Agency [APVV-0797-11]
- Grant Agency of the Slovak Republic [1/0051/13]
DOI: 10.1039/c4ay00704b
ISSN: 1759-9660
WoS: 000337763200054
Scopus: 2-s2.0-84902520872
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - Šovrc, Ľubomír AU - Stanković, Dalibor AU - Mehmeti, Eda AU - Kalcher, Kurt PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1794 AB - For the first time, a simple and sensitive analytical method for the direct determination of yohimbine is presented using differential pulse voltammetry with a boron-doped diamond electrode. Two irreversible oxidation peaks, a distinct one at +0.80 and a second poorly-defined one at +1.65 V, were observed when cyclic voltammetry was carried out in Britton-Robinson buffer solution at pH 7 (vs. Ag/AgCl). With optimized differential pulse voltammetric parameters (pulse amplitude 100 mV, pulse time 25 ms, step potential 5 mV and scan rate 10 mV s(-1)), the current response of yohimbine at +0.80 V was linearly proportional to the concentration in the range from 0.25 to 90.9 mu mol L-1 with a low detection limit of 0.13 mu mol L-1 (0.046 mg L-1) and a good repeatability (relative standard deviation of 2.5% at 18.4 mu mol L-1 for n = 6). The practical applicability of the developed method was demonstrated by the assessment of the total content of yohimbine in extracts of the primary bark of natural aphrodisiacs such as Pausinystalia yohimbe and Rauvolfia serpentina with recoveries in the range of 92-97%. The proposed electrochemical procedure represents an inexpensive and effective analytical alternative for the quality control analysis of products containing yohimbine and other biologically and structurally related alkaloids used as natural dietary supplements. PB - Royal Soc Chemistry, Cambridge T2 - Analytical Methods T1 - Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode VL - 6 IS - 13 SP - 4853 EP - 4859 DO - 10.1039/c4ay00704b ER -
@article{ author = "Šovrc, Ľubomír and Stanković, Dalibor and Mehmeti, Eda and Kalcher, Kurt", year = "2014", abstract = "For the first time, a simple and sensitive analytical method for the direct determination of yohimbine is presented using differential pulse voltammetry with a boron-doped diamond electrode. Two irreversible oxidation peaks, a distinct one at +0.80 and a second poorly-defined one at +1.65 V, were observed when cyclic voltammetry was carried out in Britton-Robinson buffer solution at pH 7 (vs. Ag/AgCl). With optimized differential pulse voltammetric parameters (pulse amplitude 100 mV, pulse time 25 ms, step potential 5 mV and scan rate 10 mV s(-1)), the current response of yohimbine at +0.80 V was linearly proportional to the concentration in the range from 0.25 to 90.9 mu mol L-1 with a low detection limit of 0.13 mu mol L-1 (0.046 mg L-1) and a good repeatability (relative standard deviation of 2.5% at 18.4 mu mol L-1 for n = 6). The practical applicability of the developed method was demonstrated by the assessment of the total content of yohimbine in extracts of the primary bark of natural aphrodisiacs such as Pausinystalia yohimbe and Rauvolfia serpentina with recoveries in the range of 92-97%. The proposed electrochemical procedure represents an inexpensive and effective analytical alternative for the quality control analysis of products containing yohimbine and other biologically and structurally related alkaloids used as natural dietary supplements.", publisher = "Royal Soc Chemistry, Cambridge", journal = "Analytical Methods", title = "Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode", volume = "6", number = "13", pages = "4853-4859", doi = "10.1039/c4ay00704b" }
Šovrc, Ľ., Stanković, D., Mehmeti, E.,& Kalcher, K.. (2014). Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode. in Analytical Methods Royal Soc Chemistry, Cambridge., 6(13), 4853-4859. https://doi.org/10.1039/c4ay00704b
Šovrc Ľ, Stanković D, Mehmeti E, Kalcher K. Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode. in Analytical Methods. 2014;6(13):4853-4859. doi:10.1039/c4ay00704b .
Šovrc, Ľubomír, Stanković, Dalibor, Mehmeti, Eda, Kalcher, Kurt, "Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode" in Analytical Methods, 6, no. 13 (2014):4853-4859, https://doi.org/10.1039/c4ay00704b . .