Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains
Abstract
Purpose: The aim of this study was to assess potential antimicrobial effectiveness of selected red wines from Balkan region, made from autochthonic Vranac V. vinifera L. grape variety. Methods: The antimicrobial activity of Vranac wines against Gram-positive: C. perfringens, B. subtillis, S. aureus, L. inocua, S. Lutea, and M. flavus and Gram-negative: E. coli, P. aeruginosa, S. enteritidis, S. sonnei, K. pneumonia and P. vulgaris bacteria stains were studied using the agar well diffusion and micro-well dilution methods. The concentrations of the wine phenolic compounds: gallic acid, caffeic acid, (+)-catechin, resveratrol, quercetin, quercetin-3-glucoside and malvidin-3-glucoside were determined using HPLC analysis. Results: There was excellent correlation between the contents of gallic acid, caffeic acid, resveratrol, quercetin, quercetin-3-glucoside and malvidine-3-glucoside and the antimicrobial activity of the wines against Gram-positive - C. perfringens and M. flavus (from 0.936 ...to 0.999) and against Gram-negative bacteria stains - E. coli, P. aeruginosa, S. enteritidis, S. sonnei and P. vulgaris (from 0.904 to 0.999). Furthermore, the content of (+)-catechin has good correlation with the antimicrobial activity of the wines only against L. inocua and P. vulgaris with correlation coefficient of 0.996 and 0.999, respectively. All selected wine phenolic compounds, however, did not show correlation with antimicrobial activity against K. pneumonia strain. Conclusion: The antimicrobial activity of selected Vranac wines indicates that some of the wine's phenolic constituents have the potential to inhibit the growth of certain bacterial strains.
Keywords:
Red wine / Antimicrobial activity / Bacterial strains / Phenolic compoundsSource:
Tropical Journal of Pharmaceutical Research, 2014, 13, 5, 819-824Publisher:
- Pharmacotherapy Group, Benin City
Funding / projects:
- Plants and synthetic bioactive products of new generation (RS-MESTD-Technological Development (TD or TR)-34012)
- Development of new technology for production of red wine and diatery supplements reach with polyphenols with cardioprotective effects (RS-MESTD-Technological Development (TD or TR)-31020)
- Geochemical investigations of sedimentary rocks - fossil fuels and environmental pollutants (RS-MESTD-Basic Research (BR or ON)-176006)
- Reinforcement of the WBC research capacities for food quality characterization (EU-FP7-204756)
- Europe Union [FP7-Regpot-2007-3-01]
DOI: 10.4314/tjpr.v13i5.24
ISSN: 1596-5996
WoS: 000339625100024
Scopus: 2-s2.0-84901914803
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Radovanović, Aleksandra AU - Jovančićević, Branimir AU - Radovanović, Blaga AU - Mihajilov-Krstev, Tatjana PY - 2014 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1822 AB - Purpose: The aim of this study was to assess potential antimicrobial effectiveness of selected red wines from Balkan region, made from autochthonic Vranac V. vinifera L. grape variety. Methods: The antimicrobial activity of Vranac wines against Gram-positive: C. perfringens, B. subtillis, S. aureus, L. inocua, S. Lutea, and M. flavus and Gram-negative: E. coli, P. aeruginosa, S. enteritidis, S. sonnei, K. pneumonia and P. vulgaris bacteria stains were studied using the agar well diffusion and micro-well dilution methods. The concentrations of the wine phenolic compounds: gallic acid, caffeic acid, (+)-catechin, resveratrol, quercetin, quercetin-3-glucoside and malvidin-3-glucoside were determined using HPLC analysis. Results: There was excellent correlation between the contents of gallic acid, caffeic acid, resveratrol, quercetin, quercetin-3-glucoside and malvidine-3-glucoside and the antimicrobial activity of the wines against Gram-positive - C. perfringens and M. flavus (from 0.936 to 0.999) and against Gram-negative bacteria stains - E. coli, P. aeruginosa, S. enteritidis, S. sonnei and P. vulgaris (from 0.904 to 0.999). Furthermore, the content of (+)-catechin has good correlation with the antimicrobial activity of the wines only against L. inocua and P. vulgaris with correlation coefficient of 0.996 and 0.999, respectively. All selected wine phenolic compounds, however, did not show correlation with antimicrobial activity against K. pneumonia strain. Conclusion: The antimicrobial activity of selected Vranac wines indicates that some of the wine's phenolic constituents have the potential to inhibit the growth of certain bacterial strains. PB - Pharmacotherapy Group, Benin City T2 - Tropical Journal of Pharmaceutical Research T1 - Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains VL - 13 IS - 5 SP - 819 EP - 824 DO - 10.4314/tjpr.v13i5.24 ER -
@article{ author = "Radovanović, Aleksandra and Jovančićević, Branimir and Radovanović, Blaga and Mihajilov-Krstev, Tatjana", year = "2014", abstract = "Purpose: The aim of this study was to assess potential antimicrobial effectiveness of selected red wines from Balkan region, made from autochthonic Vranac V. vinifera L. grape variety. Methods: The antimicrobial activity of Vranac wines against Gram-positive: C. perfringens, B. subtillis, S. aureus, L. inocua, S. Lutea, and M. flavus and Gram-negative: E. coli, P. aeruginosa, S. enteritidis, S. sonnei, K. pneumonia and P. vulgaris bacteria stains were studied using the agar well diffusion and micro-well dilution methods. The concentrations of the wine phenolic compounds: gallic acid, caffeic acid, (+)-catechin, resveratrol, quercetin, quercetin-3-glucoside and malvidin-3-glucoside were determined using HPLC analysis. Results: There was excellent correlation between the contents of gallic acid, caffeic acid, resveratrol, quercetin, quercetin-3-glucoside and malvidine-3-glucoside and the antimicrobial activity of the wines against Gram-positive - C. perfringens and M. flavus (from 0.936 to 0.999) and against Gram-negative bacteria stains - E. coli, P. aeruginosa, S. enteritidis, S. sonnei and P. vulgaris (from 0.904 to 0.999). Furthermore, the content of (+)-catechin has good correlation with the antimicrobial activity of the wines only against L. inocua and P. vulgaris with correlation coefficient of 0.996 and 0.999, respectively. All selected wine phenolic compounds, however, did not show correlation with antimicrobial activity against K. pneumonia strain. Conclusion: The antimicrobial activity of selected Vranac wines indicates that some of the wine's phenolic constituents have the potential to inhibit the growth of certain bacterial strains.", publisher = "Pharmacotherapy Group, Benin City", journal = "Tropical Journal of Pharmaceutical Research", title = "Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains", volume = "13", number = "5", pages = "819-824", doi = "10.4314/tjpr.v13i5.24" }
Radovanović, A., Jovančićević, B., Radovanović, B.,& Mihajilov-Krstev, T.. (2014). Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains. in Tropical Journal of Pharmaceutical Research Pharmacotherapy Group, Benin City., 13(5), 819-824. https://doi.org/10.4314/tjpr.v13i5.24
Radovanović A, Jovančićević B, Radovanović B, Mihajilov-Krstev T. Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains. in Tropical Journal of Pharmaceutical Research. 2014;13(5):819-824. doi:10.4314/tjpr.v13i5.24 .
Radovanović, Aleksandra, Jovančićević, Branimir, Radovanović, Blaga, Mihajilov-Krstev, Tatjana, "Antimicrobial Effectiveness of Selected Vranac Wines Against Six Gram-Positive and Six Gram-Negative Bacterial Strains" in Tropical Journal of Pharmaceutical Research, 13, no. 5 (2014):819-824, https://doi.org/10.4314/tjpr.v13i5.24 . .