New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi
Abstract
In this paper a new electrochemical method for determination of the beta-carboline alkaloids, harmalol and harmine, using differential pulse voltammetry is presented. Experimental parameters such as pH, scan rate, pulse amplitude and pulse time were optimized to characterize their electrochemical behavior and to find best analytical conditions. Thus, in the presence of ascorbic acid, uric acid, dopamine, the developed method exhibits excellent performance for the determination of harmalol and harmine for a linear concentration range between 1-50 mu M and 1-75 mu M with a detection limit of 0.6 mu M and 0.2 mu M, respectively. The proposed method was successfully applied for the determination of these alkaloids in a sample of an Ayahuasca liana (Banisteriopsis caapi) and in model human urine samples with good recoveries.
Keywords:
Harmalol / Harmine / Banisteriopsis caapi / Differential pulse voltammetrySource:
Microchemical Journal, 2015, 118, 95-100Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Austrian government
- Grant Agency of the Slovak Republic [1/0051/13]
DOI: 10.1016/j.microc.2014.08.007
ISSN: 0026-265X
WoS: 000345733900014
Scopus: 2-s2.0-84927590854
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Stanković, Dalibor AU - Mehmeti, Eda AU - Šovrc, Ľubomír AU - Kalcher, Kurt PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1884 AB - In this paper a new electrochemical method for determination of the beta-carboline alkaloids, harmalol and harmine, using differential pulse voltammetry is presented. Experimental parameters such as pH, scan rate, pulse amplitude and pulse time were optimized to characterize their electrochemical behavior and to find best analytical conditions. Thus, in the presence of ascorbic acid, uric acid, dopamine, the developed method exhibits excellent performance for the determination of harmalol and harmine for a linear concentration range between 1-50 mu M and 1-75 mu M with a detection limit of 0.6 mu M and 0.2 mu M, respectively. The proposed method was successfully applied for the determination of these alkaloids in a sample of an Ayahuasca liana (Banisteriopsis caapi) and in model human urine samples with good recoveries. PB - Elsevier Science Bv, Amsterdam T2 - Microchemical Journal T1 - New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi VL - 118 SP - 95 EP - 100 DO - 10.1016/j.microc.2014.08.007 ER -
@article{ author = "Stanković, Dalibor and Mehmeti, Eda and Šovrc, Ľubomír and Kalcher, Kurt", year = "2015", abstract = "In this paper a new electrochemical method for determination of the beta-carboline alkaloids, harmalol and harmine, using differential pulse voltammetry is presented. Experimental parameters such as pH, scan rate, pulse amplitude and pulse time were optimized to characterize their electrochemical behavior and to find best analytical conditions. Thus, in the presence of ascorbic acid, uric acid, dopamine, the developed method exhibits excellent performance for the determination of harmalol and harmine for a linear concentration range between 1-50 mu M and 1-75 mu M with a detection limit of 0.6 mu M and 0.2 mu M, respectively. The proposed method was successfully applied for the determination of these alkaloids in a sample of an Ayahuasca liana (Banisteriopsis caapi) and in model human urine samples with good recoveries.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Microchemical Journal", title = "New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi", volume = "118", pages = "95-100", doi = "10.1016/j.microc.2014.08.007" }
Stanković, D., Mehmeti, E., Šovrc, Ľ.,& Kalcher, K.. (2015). New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi. in Microchemical Journal Elsevier Science Bv, Amsterdam., 118, 95-100. https://doi.org/10.1016/j.microc.2014.08.007
Stanković D, Mehmeti E, Šovrc Ľ, Kalcher K. New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi. in Microchemical Journal. 2015;118:95-100. doi:10.1016/j.microc.2014.08.007 .
Stanković, Dalibor, Mehmeti, Eda, Šovrc, Ľubomír, Kalcher, Kurt, "New electrochemical method for the determination of beta-carboline alkaloids, harmalol and harmine, in human urine samples and in Banisteriopsis caapi" in Microchemical Journal, 118 (2015):95-100, https://doi.org/10.1016/j.microc.2014.08.007 . .