Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate
Апстракт
Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient ...in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.
Кључне речи:
alpha-amylase / glucoamylase / fungi / enzyme production / solid-state fermentation / raw starchИзвор:
Journal of the Serbian Chemical Society, 2015, 80, 11, 1375-1390Издавач:
- Serbian Chemical Soc, Belgrade
Финансирање / пројекти:
DOI: 10.2298/JSC150317041D
ISSN: 0352-5139
WoS: 000366585800004
Scopus: 2-s2.0-84957599434
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Dojnov, Biljana AU - Grujić, Marica AU - Vujčić, Zoran PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2011 AB - Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate VL - 80 IS - 11 SP - 1375 EP - 1390 DO - 10.2298/JSC150317041D ER -
@article{ author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran", year = "2015", abstract = "Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate", volume = "80", number = "11", pages = "1375-1390", doi = "10.2298/JSC150317041D" }
Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 80(11), 1375-1390. https://doi.org/10.2298/JSC150317041D
Dojnov B, Grujić M, Vujčić Z. Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society. 2015;80(11):1375-1390. doi:10.2298/JSC150317041D .
Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1375-1390, https://doi.org/10.2298/JSC150317041D . .