An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles
Samo za registrovane korisnike
2015
Autori
Samphao, AnchaleeKunpatee, Kanjana
Prayoonpokarach, Sanchai
Wittayakun, Jatuporn
Šovrc, Ľubomír
Stanković, Dalibor
Zagar, Kristina
Ceh, Miran
Kalcher, Kurt
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4"Au NPs) is presented. ADH was immobilized on Fe3O4"Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4"Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determinati...on of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%.
Ključne reči:
Magnetic-gold nanoparticles / ethanol biosensor / carbon paste electrode / ethanol determination / alcohol dehydrogenaseIzvor:
Electroanalysis, 2015, 27, 12, 2829-2837Izdavač:
- Wiley-V C H Verlag Gmbh, Weinheim
Finansiranje / projekti:
- AseaUniNet
- Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ubon Ratchathani University
- National Research Council of Thailand (NRCT)
- Department of Chemistry, Faculty of Science, Ubon Ratchathani University (Thailand)
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3331
DOI: 10.1002/elan.201500315
ISSN: 1040-0397
WoS: 000368340300018
Scopus: 2-s2.0-84954399331
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - Samphao, Anchalee AU - Kunpatee, Kanjana AU - Prayoonpokarach, Sanchai AU - Wittayakun, Jatuporn AU - Šovrc, Ľubomír AU - Stanković, Dalibor AU - Zagar, Kristina AU - Ceh, Miran AU - Kalcher, Kurt PY - 2015 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2034 AB - An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4"Au NPs) is presented. ADH was immobilized on Fe3O4"Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4"Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determination of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%. PB - Wiley-V C H Verlag Gmbh, Weinheim T2 - Electroanalysis T1 - An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles VL - 27 IS - 12 SP - 2829 EP - 2837 DO - 10.1002/elan.201500315 ER -
@article{ author = "Samphao, Anchalee and Kunpatee, Kanjana and Prayoonpokarach, Sanchai and Wittayakun, Jatuporn and Šovrc, Ľubomír and Stanković, Dalibor and Zagar, Kristina and Ceh, Miran and Kalcher, Kurt", year = "2015", abstract = "An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4"Au NPs) is presented. ADH was immobilized on Fe3O4"Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4"Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determination of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%.", publisher = "Wiley-V C H Verlag Gmbh, Weinheim", journal = "Electroanalysis", title = "An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles", volume = "27", number = "12", pages = "2829-2837", doi = "10.1002/elan.201500315" }
Samphao, A., Kunpatee, K., Prayoonpokarach, S., Wittayakun, J., Šovrc, Ľ., Stanković, D., Zagar, K., Ceh, M.,& Kalcher, K.. (2015). An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. in Electroanalysis Wiley-V C H Verlag Gmbh, Weinheim., 27(12), 2829-2837. https://doi.org/10.1002/elan.201500315
Samphao A, Kunpatee K, Prayoonpokarach S, Wittayakun J, Šovrc Ľ, Stanković D, Zagar K, Ceh M, Kalcher K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. in Electroanalysis. 2015;27(12):2829-2837. doi:10.1002/elan.201500315 .
Samphao, Anchalee, Kunpatee, Kanjana, Prayoonpokarach, Sanchai, Wittayakun, Jatuporn, Šovrc, Ľubomír, Stanković, Dalibor, Zagar, Kristina, Ceh, Miran, Kalcher, Kurt, "An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles" in Electroanalysis, 27, no. 12 (2015):2829-2837, https://doi.org/10.1002/elan.201500315 . .