Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats
Uticaj atorvastatina i tinkture lista artičoke na oksidativni stres kod pacova sa hiperholesterolemijom
2016
Аутори
Crevar-Sakač, MilkicaVujić, Zorica
Kotur-Stevuljević, Jelena
Ivanišević, Jasmina
Jelić-Ivanović, Zorana
Milenković, Marina
Markelic, Milica
Vujčić, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Backgroung/Aim. Since combining conventional drugs with herbal medicinal products is in current research focus and possible of great interest as therapy improvement way, the aim of this study was to determine the effects of well established antiatherosclerotic drug atorvastatin (CAS number 134523-00-5) and commercially available artichoke leaf tincture (ALTINC), used as combined therapy, as well as to compare effects of these two treatments separately. Methods. Experimental animals were divided into five groups: the group I (the control group of rats fed with standard diet during 11 weeks), and the remaining 4 groups of rats (II, III, IV and V) fed with standard diet during the first week and then with hypercholesterolemic diet during the next 10 weeks. The group II of rats were left without treatment, while in the groups III, IV and V were rats treated per as with atorvastatin (1.15 mg/kg body wright - b.w.), ALTINC (0.1 mL/kg b.w.) and their combination in same doses, respectively, f...or the last six weeks. Results. The cholesterol rich diet led to pronounced hyperlipidemia which could not be overcame with the therapy. However, the therapy showed positive effects on abdominal aorta wall thickness and parameters of oxidative stress (malondialdehyde MDA, proxidative-antioxidative balance - PAB) and anti oxidative protection (reduced glutathione - GSH, paraoxanase 1 - PON1, superoxide dismutase - SODA SH groups), especially ALTINC was successful in oxidative status improvement. Conclusion. Separate treatments comparison showed that artichoke leaf tincture is very potent antioxidant with beneficial effects in early stages of atherosclerosis. Since atorvastatin and constituents of ALTINC probably have different mechanisms of action, simultaneous use of both therapies could be beneficial but should be further investigated since our results showed that ALTINC is less effective when used in combination with atorvastatin.
Uvod/Cilj. Savremena istraživanja sve više se okreću mogućnosti kombinovanja konvencionalne terapije sa biljnim lekovitim proizvodima. Cilj ovog istraživanja bio je praćenje efekata atorvastatina (CAS broj 134523-00-5), poznatog leka koji se koristi u terapiji hiperlipidemije, i tinkture lista artičoke primenjenih pojedinačno, kao i u obliku kombinovane terapije. Metode. Eksperimentalne životinje bile su podeljene u pet grupa: grupa I bila je kontrolna i činili su je pacovi hranjeni standardnom hranom za glodare tokom 11 nedelja, dok su preostale četiri grupe (II-V) činili pacovi koji su hranjeni standardnom hranom tokom prve nedelje, a potom u narednih 10 hranom bogatom holesterolom. Grupa II nije dobijala nikakav tretman, dok su III, IV i V posle 4. nedelje od početka uzimanja hrane bogate holesterolom, tokom narednih šest nedelja tretirane per os, redom: atorvastatinom u dozi od 1,15 mg/kg, tinkturom artičoke u dozi od 0,1 mL/kg i njihovom kombinacijom u istim dozama. Rezultati. Hra...na bogata holesterolom izazvala je uznapredovalu hiperholesterolemiju na koju terapija nije značajno delovala. Terapija je pokazala povoljan efekat na debljinu zida trbušne aorte, parametre oksidativnog stresa (malondialdehid - MDA, prooksidativni-antioksidativni balans - PAB) i anti-oksidativne zaštite (redukovani glutation - GSH, SH grupe, paraoksonazu1 - PON1, superoksid dismutazu - SOD). Tinktura lista artičoke pokazala se najboljom u poboljšanju oksidativnog statusa. Zaključak. Poređenje pojedinačne terapije pokazalo je da tinktura lista artičoke ima jako anti-oksidativno dejstvo i pokazuje povoljne efekte u početnim fazama aterosklerotskog procesa. S obzirom na to da atorvastatin i komponente tinkture lista artičoke verovatno deluju različitim mehanizmima, kombinovana primena može imati povoljne efekte, ali se mora dodatno ispitati jer dobijeni rezultati ukazuju da je tinktura lista artičoke manje efikasan kada se primenjuje zajedno sa atorvastatinom.
Кључне речи:
cynara scolimus / atorvastatin calcium / atorvastatin calcium / atherosclerosis / atherosclerosis / oxidative stress / oxidative stress / rats / rats / treatment outcome / treatment outcome / cynara scolimus / atorvastatin kalcijum / ateroskleroza / stres / oksidativni / pacovi / lečenje / ishodИзвор:
Vojnosanitetski pregled, 2016, 73, 2, 178-187Издавач:
- Military Medical Acad-Ini, Belgrade
Финансирање / пројекти:
- Интерактивна улога дислипидемије, оксидативног стреса и инфламације у атеросклерози и другим болестима: генетички и биохемијски маркери (RS-MESTD-Basic Research (BR or ON)-175035)
- Развој молекула са антиинфламаторним и кардиопроактивним дејством: структурне модификације, моделовање, физичкохемијска карактеризација и формулациона испитивања (RS-MESTD-Basic Research (BR or ON)-172041)
DOI: 10.2298/VSP140917148C
ISSN: 0042-8450
PubMed: 27071286
WoS: 000372045200008
Scopus: 2-s2.0-84957956200
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Crevar-Sakač, Milkica AU - Vujić, Zorica AU - Kotur-Stevuljević, Jelena AU - Ivanišević, Jasmina AU - Jelić-Ivanović, Zorana AU - Milenković, Marina AU - Markelic, Milica AU - Vujčić, Zoran PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2064 AB - Backgroung/Aim. Since combining conventional drugs with herbal medicinal products is in current research focus and possible of great interest as therapy improvement way, the aim of this study was to determine the effects of well established antiatherosclerotic drug atorvastatin (CAS number 134523-00-5) and commercially available artichoke leaf tincture (ALTINC), used as combined therapy, as well as to compare effects of these two treatments separately. Methods. Experimental animals were divided into five groups: the group I (the control group of rats fed with standard diet during 11 weeks), and the remaining 4 groups of rats (II, III, IV and V) fed with standard diet during the first week and then with hypercholesterolemic diet during the next 10 weeks. The group II of rats were left without treatment, while in the groups III, IV and V were rats treated per as with atorvastatin (1.15 mg/kg body wright - b.w.), ALTINC (0.1 mL/kg b.w.) and their combination in same doses, respectively, for the last six weeks. Results. The cholesterol rich diet led to pronounced hyperlipidemia which could not be overcame with the therapy. However, the therapy showed positive effects on abdominal aorta wall thickness and parameters of oxidative stress (malondialdehyde MDA, proxidative-antioxidative balance - PAB) and anti oxidative protection (reduced glutathione - GSH, paraoxanase 1 - PON1, superoxide dismutase - SODA SH groups), especially ALTINC was successful in oxidative status improvement. Conclusion. Separate treatments comparison showed that artichoke leaf tincture is very potent antioxidant with beneficial effects in early stages of atherosclerosis. Since atorvastatin and constituents of ALTINC probably have different mechanisms of action, simultaneous use of both therapies could be beneficial but should be further investigated since our results showed that ALTINC is less effective when used in combination with atorvastatin. AB - Uvod/Cilj. Savremena istraživanja sve više se okreću mogućnosti kombinovanja konvencionalne terapije sa biljnim lekovitim proizvodima. Cilj ovog istraživanja bio je praćenje efekata atorvastatina (CAS broj 134523-00-5), poznatog leka koji se koristi u terapiji hiperlipidemije, i tinkture lista artičoke primenjenih pojedinačno, kao i u obliku kombinovane terapije. Metode. Eksperimentalne životinje bile su podeljene u pet grupa: grupa I bila je kontrolna i činili su je pacovi hranjeni standardnom hranom za glodare tokom 11 nedelja, dok su preostale četiri grupe (II-V) činili pacovi koji su hranjeni standardnom hranom tokom prve nedelje, a potom u narednih 10 hranom bogatom holesterolom. Grupa II nije dobijala nikakav tretman, dok su III, IV i V posle 4. nedelje od početka uzimanja hrane bogate holesterolom, tokom narednih šest nedelja tretirane per os, redom: atorvastatinom u dozi od 1,15 mg/kg, tinkturom artičoke u dozi od 0,1 mL/kg i njihovom kombinacijom u istim dozama. Rezultati. Hrana bogata holesterolom izazvala je uznapredovalu hiperholesterolemiju na koju terapija nije značajno delovala. Terapija je pokazala povoljan efekat na debljinu zida trbušne aorte, parametre oksidativnog stresa (malondialdehid - MDA, prooksidativni-antioksidativni balans - PAB) i anti-oksidativne zaštite (redukovani glutation - GSH, SH grupe, paraoksonazu1 - PON1, superoksid dismutazu - SOD). Tinktura lista artičoke pokazala se najboljom u poboljšanju oksidativnog statusa. Zaključak. Poređenje pojedinačne terapije pokazalo je da tinktura lista artičoke ima jako anti-oksidativno dejstvo i pokazuje povoljne efekte u početnim fazama aterosklerotskog procesa. S obzirom na to da atorvastatin i komponente tinkture lista artičoke verovatno deluju različitim mehanizmima, kombinovana primena može imati povoljne efekte, ali se mora dodatno ispitati jer dobijeni rezultati ukazuju da je tinktura lista artičoke manje efikasan kada se primenjuje zajedno sa atorvastatinom. PB - Military Medical Acad-Ini, Belgrade T2 - Vojnosanitetski pregled T1 - Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats T1 - Uticaj atorvastatina i tinkture lista artičoke na oksidativni stres kod pacova sa hiperholesterolemijom VL - 73 IS - 2 SP - 178 EP - 187 DO - 10.2298/VSP140917148C ER -
@article{ author = "Crevar-Sakač, Milkica and Vujić, Zorica and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Jelić-Ivanović, Zorana and Milenković, Marina and Markelic, Milica and Vujčić, Zoran", year = "2016", abstract = "Backgroung/Aim. Since combining conventional drugs with herbal medicinal products is in current research focus and possible of great interest as therapy improvement way, the aim of this study was to determine the effects of well established antiatherosclerotic drug atorvastatin (CAS number 134523-00-5) and commercially available artichoke leaf tincture (ALTINC), used as combined therapy, as well as to compare effects of these two treatments separately. Methods. Experimental animals were divided into five groups: the group I (the control group of rats fed with standard diet during 11 weeks), and the remaining 4 groups of rats (II, III, IV and V) fed with standard diet during the first week and then with hypercholesterolemic diet during the next 10 weeks. The group II of rats were left without treatment, while in the groups III, IV and V were rats treated per as with atorvastatin (1.15 mg/kg body wright - b.w.), ALTINC (0.1 mL/kg b.w.) and their combination in same doses, respectively, for the last six weeks. Results. The cholesterol rich diet led to pronounced hyperlipidemia which could not be overcame with the therapy. However, the therapy showed positive effects on abdominal aorta wall thickness and parameters of oxidative stress (malondialdehyde MDA, proxidative-antioxidative balance - PAB) and anti oxidative protection (reduced glutathione - GSH, paraoxanase 1 - PON1, superoxide dismutase - SODA SH groups), especially ALTINC was successful in oxidative status improvement. Conclusion. Separate treatments comparison showed that artichoke leaf tincture is very potent antioxidant with beneficial effects in early stages of atherosclerosis. Since atorvastatin and constituents of ALTINC probably have different mechanisms of action, simultaneous use of both therapies could be beneficial but should be further investigated since our results showed that ALTINC is less effective when used in combination with atorvastatin., Uvod/Cilj. Savremena istraživanja sve više se okreću mogućnosti kombinovanja konvencionalne terapije sa biljnim lekovitim proizvodima. Cilj ovog istraživanja bio je praćenje efekata atorvastatina (CAS broj 134523-00-5), poznatog leka koji se koristi u terapiji hiperlipidemije, i tinkture lista artičoke primenjenih pojedinačno, kao i u obliku kombinovane terapije. Metode. Eksperimentalne životinje bile su podeljene u pet grupa: grupa I bila je kontrolna i činili su je pacovi hranjeni standardnom hranom za glodare tokom 11 nedelja, dok su preostale četiri grupe (II-V) činili pacovi koji su hranjeni standardnom hranom tokom prve nedelje, a potom u narednih 10 hranom bogatom holesterolom. Grupa II nije dobijala nikakav tretman, dok su III, IV i V posle 4. nedelje od početka uzimanja hrane bogate holesterolom, tokom narednih šest nedelja tretirane per os, redom: atorvastatinom u dozi od 1,15 mg/kg, tinkturom artičoke u dozi od 0,1 mL/kg i njihovom kombinacijom u istim dozama. Rezultati. Hrana bogata holesterolom izazvala je uznapredovalu hiperholesterolemiju na koju terapija nije značajno delovala. Terapija je pokazala povoljan efekat na debljinu zida trbušne aorte, parametre oksidativnog stresa (malondialdehid - MDA, prooksidativni-antioksidativni balans - PAB) i anti-oksidativne zaštite (redukovani glutation - GSH, SH grupe, paraoksonazu1 - PON1, superoksid dismutazu - SOD). Tinktura lista artičoke pokazala se najboljom u poboljšanju oksidativnog statusa. Zaključak. Poređenje pojedinačne terapije pokazalo je da tinktura lista artičoke ima jako anti-oksidativno dejstvo i pokazuje povoljne efekte u početnim fazama aterosklerotskog procesa. S obzirom na to da atorvastatin i komponente tinkture lista artičoke verovatno deluju različitim mehanizmima, kombinovana primena može imati povoljne efekte, ali se mora dodatno ispitati jer dobijeni rezultati ukazuju da je tinktura lista artičoke manje efikasan kada se primenjuje zajedno sa atorvastatinom.", publisher = "Military Medical Acad-Ini, Belgrade", journal = "Vojnosanitetski pregled", title = "Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats, Uticaj atorvastatina i tinkture lista artičoke na oksidativni stres kod pacova sa hiperholesterolemijom", volume = "73", number = "2", pages = "178-187", doi = "10.2298/VSP140917148C" }
Crevar-Sakač, M., Vujić, Z., Kotur-Stevuljević, J., Ivanišević, J., Jelić-Ivanović, Z., Milenković, M., Markelic, M.,& Vujčić, Z.. (2016). Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats. in Vojnosanitetski pregled Military Medical Acad-Ini, Belgrade., 73(2), 178-187. https://doi.org/10.2298/VSP140917148C
Crevar-Sakač M, Vujić Z, Kotur-Stevuljević J, Ivanišević J, Jelić-Ivanović Z, Milenković M, Markelic M, Vujčić Z. Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats. in Vojnosanitetski pregled. 2016;73(2):178-187. doi:10.2298/VSP140917148C .
Crevar-Sakač, Milkica, Vujić, Zorica, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Jelić-Ivanović, Zorana, Milenković, Marina, Markelic, Milica, Vujčić, Zoran, "Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats" in Vojnosanitetski pregled, 73, no. 2 (2016):178-187, https://doi.org/10.2298/VSP140917148C . .