NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells
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2018
Authors
Mančić, LidijaĐukić-Vuković, Aleksandra
Dinić, Ivana
Nikolić, Marko G.
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
Costa, Antonio M. L. M.
Trišić, Dijana
Lazarević, Miloš
Mojović, Ljiljana
Milošević, Olivera B.
Article (Published version)
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Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, res...pectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.
Keywords:
Upconversion nanoparticles / PLGA / NaYF4:Yb,Er / Bioimaging / Cancer cell / Human gingival cellSource:
Materials Science and Engineering. C: Materials for Biological Applications, 2018, 91, 597-605Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-MESTD-Basic Research (BR or ON)-172035)
- Fabrication and characterization of nano-photonic functional structrues in biomedicine and informatics (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45016)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3003
DOI: 10.1016/j.msec.2018.05.081
ISSN: 0928-4931
PubMed: 30033292
WoS: 000442192000061
Scopus: 2-s2.0-85047855276
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Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Mančić, Lidija AU - Đukić-Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar J. AU - Costa, Antonio M. L. M. AU - Trišić, Dijana AU - Lazarević, Miloš AU - Mojović, Ljiljana AU - Milošević, Olivera B. PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2205 AB - Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy. PB - Elsevier Science Bv, Amsterdam T2 - Materials Science and Engineering. C: Materials for Biological Applications T1 - NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells VL - 91 SP - 597 EP - 605 DO - 10.1016/j.msec.2018.05.081 ER -
@article{ author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Costa, Antonio M. L. M. and Trišić, Dijana and Lazarević, Miloš and Mojović, Ljiljana and Milošević, Olivera B.", year = "2018", abstract = "Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Materials Science and Engineering. C: Materials for Biological Applications", title = "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells", volume = "91", pages = "597-605", doi = "10.1016/j.msec.2018.05.081" }
Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A. J., Costa, A. M. L. M., Trišić, D., Lazarević, M., Mojović, L.,& Milošević, O. B.. (2018). NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science and Engineering. C: Materials for Biological Applications Elsevier Science Bv, Amsterdam., 91, 597-605. https://doi.org/10.1016/j.msec.2018.05.081
Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot AJ, Costa AMLM, Trišić D, Lazarević M, Mojović L, Milošević OB. NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science and Engineering. C: Materials for Biological Applications. 2018;91:597-605. doi:10.1016/j.msec.2018.05.081 .
Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar J., Costa, Antonio M. L. M., Trišić, Dijana, Lazarević, Miloš, Mojović, Ljiljana, Milošević, Olivera B., "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells" in Materials Science and Engineering. C: Materials for Biological Applications, 91 (2018):597-605, https://doi.org/10.1016/j.msec.2018.05.081 . .