One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging
2018
Autori
Mančić, LidijaĐukić-Vuković, Aleksandra
Dinić, Ivana
Nikolić, Marko G.
Rabasović, Mihailo D.
Krmpot, Aleksandar J.
Costa, Antonio M. L. M.
Marinkovic, Bojan A.
Mojović, Ljiljana
Milošević, Olivera B.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result ...of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.
Izvor:
RSC Advances, 2018, 8, 48, 27429-27437Izdavač:
- Royal Soc Chemistry, Cambridge
Finansiranje / projekti:
- Racionalni dizajn i sinteza biološki aktivnih i koordinacionih jedinjenja i funkcionalnih materijala, relevantnih u (bio)nanotehnologiji (RS-172035)
- Generisanje i karakterizacija nanofotonskih funkcionalnih struktura u biomedicini i informatici (RS-45016)
- CNPq (National Council for Scientific and Technological Development)
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3132
DOI: 10.1039/c8ra04178d
ISSN: 2046-2069
WoS: 000445747800050
Scopus: 2-s2.0-85051345804
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - Mančić, Lidija AU - Đukić-Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar J. AU - Costa, Antonio M. L. M. AU - Marinkovic, Bojan A. AU - Mojović, Ljiljana AU - Milošević, Olivera B. PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2225 AB - The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. PB - Royal Soc Chemistry, Cambridge T2 - RSC Advances T1 - One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging VL - 8 IS - 48 SP - 27429 EP - 27437 DO - 10.1039/c8ra04178d ER -
@article{ author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar J. and Costa, Antonio M. L. M. and Marinkovic, Bojan A. and Mojović, Ljiljana and Milošević, Olivera B.", year = "2018", abstract = "The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt (4)/(15/2)) and stronger red emission (F-4(9/2) - gt (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.", publisher = "Royal Soc Chemistry, Cambridge", journal = "RSC Advances", title = "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging", volume = "8", number = "48", pages = "27429-27437", doi = "10.1039/c8ra04178d" }
Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A. J., Costa, A. M. L. M., Marinkovic, B. A., Mojović, L.,& Milošević, O. B.. (2018). One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances Royal Soc Chemistry, Cambridge., 8(48), 27429-27437. https://doi.org/10.1039/c8ra04178d
Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot AJ, Costa AMLM, Marinkovic BA, Mojović L, Milošević OB. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances. 2018;8(48):27429-27437. doi:10.1039/c8ra04178d .
Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar J., Costa, Antonio M. L. M., Marinkovic, Bojan A., Mojović, Ljiljana, Milošević, Olivera B., "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging" in RSC Advances, 8, no. 48 (2018):27429-27437, https://doi.org/10.1039/c8ra04178d . .