Development of RAST assay for determination of anti-Populus canadensis IgE antibodies
Апстракт
One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.
Извор:
Journal of Radioanalytical and Nuclear Chemistry, 1996, 206, 1, 145-149Издавач:
- Akademiai Kiado, Budapest
DOI: 10.1007/BF02040051
ISSN: 0236-5731
WoS: A1996VD12500016
Scopus: 2-s2.0-0029834625
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Trtic, T AU - Vuksanovic, L AU - Paranos, S AU - Petrović, S. AU - Vujčić, Zoran AU - Plecas, I AU - Jankov, Ratko M. PY - 1996 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2246 AB - One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides. PB - Akademiai Kiado, Budapest T2 - Journal of Radioanalytical and Nuclear Chemistry T1 - Development of RAST assay for determination of anti-Populus canadensis IgE antibodies VL - 206 IS - 1 SP - 145 EP - 149 DO - 10.1007/BF02040051 ER -
@article{ author = "Trtic, T and Vuksanovic, L and Paranos, S and Petrović, S. and Vujčić, Zoran and Plecas, I and Jankov, Ratko M.", year = "1996", abstract = "One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.", publisher = "Akademiai Kiado, Budapest", journal = "Journal of Radioanalytical and Nuclear Chemistry", title = "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies", volume = "206", number = "1", pages = "145-149", doi = "10.1007/BF02040051" }
Trtic, T., Vuksanovic, L., Paranos, S., Petrović, S., Vujčić, Z., Plecas, I.,& Jankov, R. M.. (1996). Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry Akademiai Kiado, Budapest., 206(1), 145-149. https://doi.org/10.1007/BF02040051
Trtic T, Vuksanovic L, Paranos S, Petrović S, Vujčić Z, Plecas I, Jankov RM. Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry. 1996;206(1):145-149. doi:10.1007/BF02040051 .
Trtic, T, Vuksanovic, L, Paranos, S, Petrović, S., Vujčić, Zoran, Plecas, I, Jankov, Ratko M., "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies" in Journal of Radioanalytical and Nuclear Chemistry, 206, no. 1 (1996):145-149, https://doi.org/10.1007/BF02040051 . .