Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles
Abstract
A simple, sensitive, and economically viable electrochemical method for the determination of ajmalicine was developed. The procedure involves a glassy carbon electrode modified with gold nanoparticles (GCE/AuNPs). In the work presented here gold nanoparticles were attached to the glassy carbon electrode by adsorption. Cyclic voltammetry was used for the first time for the electrochemical characterization of ajmalicine and differential pulse voltammetry for its quantitative determination. Britton-Robinson buffer solution in a range of pH values served as supporting electrolyte. The experimental conditions were optimized and it was found that ajmalicine provided a well-defined oxidation peak at a potential of +0.8 V vs. Ag/AgCl (3 M KCl). The method for the determination of ajmalicine showed a dynamic range for concentrations from 5 to 50 A mu M with a detection limit of 1.7 A mu M, good repeatability (RSD of 1.4 %) and reproducibility (RSD of 2.5 %). The effect of possible interferents ...(yohimbine, reserpine) appeared to be negligible. The proposed procedure was successfully applied for the first time to the determination of ajmalicine in commercially available oral solutions for medical applications with results in good agreement with the declared content of ajmalicine. [GRAPHICS]
Keywords:
Alkaloids / Cyclic voltammetry / Electrochemistry / Natural products / Gold nanoparticlesSource:
Monatshefte Fur Chemie, 2016, 147, 7, 1161-1166Publisher:
- Springer Wien, Wien
Funding / projects:
- Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-MESTD-Basic Research (BR or ON)-172030)
- Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH)
- Higher KOS Stipendien - ADA
DOI: 10.1007/s00706-016-1741-7
ISSN: 0026-9247
WoS: 000377429800003
Scopus: 2-s2.0-84962200885
Collections
Institution/Community
Inovacioni centar / Innovation CentreTY - JOUR AU - Mehmeti, Eda AU - Stanković, Dalibor AU - Chaiyo, Sudkate AU - Šovrc, Ľubomír AU - Ortner, Astrid AU - Kalcher, Kurt PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2259 AB - A simple, sensitive, and economically viable electrochemical method for the determination of ajmalicine was developed. The procedure involves a glassy carbon electrode modified with gold nanoparticles (GCE/AuNPs). In the work presented here gold nanoparticles were attached to the glassy carbon electrode by adsorption. Cyclic voltammetry was used for the first time for the electrochemical characterization of ajmalicine and differential pulse voltammetry for its quantitative determination. Britton-Robinson buffer solution in a range of pH values served as supporting electrolyte. The experimental conditions were optimized and it was found that ajmalicine provided a well-defined oxidation peak at a potential of +0.8 V vs. Ag/AgCl (3 M KCl). The method for the determination of ajmalicine showed a dynamic range for concentrations from 5 to 50 A mu M with a detection limit of 1.7 A mu M, good repeatability (RSD of 1.4 %) and reproducibility (RSD of 2.5 %). The effect of possible interferents (yohimbine, reserpine) appeared to be negligible. The proposed procedure was successfully applied for the first time to the determination of ajmalicine in commercially available oral solutions for medical applications with results in good agreement with the declared content of ajmalicine. [GRAPHICS] PB - Springer Wien, Wien T2 - Monatshefte Fur Chemie T1 - Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles VL - 147 IS - 7 SP - 1161 EP - 1166 DO - 10.1007/s00706-016-1741-7 ER -
@article{ author = "Mehmeti, Eda and Stanković, Dalibor and Chaiyo, Sudkate and Šovrc, Ľubomír and Ortner, Astrid and Kalcher, Kurt", year = "2016", abstract = "A simple, sensitive, and economically viable electrochemical method for the determination of ajmalicine was developed. The procedure involves a glassy carbon electrode modified with gold nanoparticles (GCE/AuNPs). In the work presented here gold nanoparticles were attached to the glassy carbon electrode by adsorption. Cyclic voltammetry was used for the first time for the electrochemical characterization of ajmalicine and differential pulse voltammetry for its quantitative determination. Britton-Robinson buffer solution in a range of pH values served as supporting electrolyte. The experimental conditions were optimized and it was found that ajmalicine provided a well-defined oxidation peak at a potential of +0.8 V vs. Ag/AgCl (3 M KCl). The method for the determination of ajmalicine showed a dynamic range for concentrations from 5 to 50 A mu M with a detection limit of 1.7 A mu M, good repeatability (RSD of 1.4 %) and reproducibility (RSD of 2.5 %). The effect of possible interferents (yohimbine, reserpine) appeared to be negligible. The proposed procedure was successfully applied for the first time to the determination of ajmalicine in commercially available oral solutions for medical applications with results in good agreement with the declared content of ajmalicine. [GRAPHICS]", publisher = "Springer Wien, Wien", journal = "Monatshefte Fur Chemie", title = "Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles", volume = "147", number = "7", pages = "1161-1166", doi = "10.1007/s00706-016-1741-7" }
Mehmeti, E., Stanković, D., Chaiyo, S., Šovrc, Ľ., Ortner, A.,& Kalcher, K.. (2016). Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles. in Monatshefte Fur Chemie Springer Wien, Wien., 147(7), 1161-1166. https://doi.org/10.1007/s00706-016-1741-7
Mehmeti E, Stanković D, Chaiyo S, Šovrc Ľ, Ortner A, Kalcher K. Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles. in Monatshefte Fur Chemie. 2016;147(7):1161-1166. doi:10.1007/s00706-016-1741-7 .
Mehmeti, Eda, Stanković, Dalibor, Chaiyo, Sudkate, Šovrc, Ľubomír, Ortner, Astrid, Kalcher, Kurt, "Electrochemical determination of ajmalicine using glassy carbon electrode modified with gold nanoparticles" in Monatshefte Fur Chemie, 147, no. 7 (2016):1161-1166, https://doi.org/10.1007/s00706-016-1741-7 . .