Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone
Apstrakt
Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino) benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect (CPE) in Hep-2 cells. Cells that were exposed to the 19 mu M ligand before infection displayed a maximal significant reduction (by 24.42 +/- 1.49%) of the CPE. This was likely due to the inhibition of virus receptors and prevention of viral adsorption. Tre...atment with 17 mu M Pt(II) complex after viral infection caused a maximal significant reduction (by 30.52 +/- 3.12%) of the CPE, presumably through an effect on viral replication. The results indicate that the ligand should be viewed as a potential anticancer agent. The ligand and the Pt(II) complex show promising results for further investigation of antiviral activity.
Ključne reči:
Cytotoxicity / antiviral activity / 2-(diphenylphosphino)benzaldehyde hydrazone ligands / cancer cell lines / apoptosisIzvor:
Turkish Journal of Biology, 2016, 40, 3, 661-669Izdavač:
- Tubitak Scientific & Technical Research Council Turkey, Ankara
Finansiranje / projekti:
- Biološki aktivni prirodni proizvodi kao potencijalni izvori novih lekova i dijetetskih suplemenata (RS-MESTD-Basic Research (BR or ON)-172058)
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-MESTD-Basic Research (BR or ON)-172017)
DOI: 10.3906/biy-1503-23
ISSN: 1300-0152
WoS: 000378689400015
Scopus: 2-s2.0-84969540550
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Simic, Vera AU - Kolarević, Stoimir AU - Brčeski, Ilija AU - Jeremić, Dejan AU - Vuković-Gačić, Branka PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2269 AB - Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino) benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect (CPE) in Hep-2 cells. Cells that were exposed to the 19 mu M ligand before infection displayed a maximal significant reduction (by 24.42 +/- 1.49%) of the CPE. This was likely due to the inhibition of virus receptors and prevention of viral adsorption. Treatment with 17 mu M Pt(II) complex after viral infection caused a maximal significant reduction (by 30.52 +/- 3.12%) of the CPE, presumably through an effect on viral replication. The results indicate that the ligand should be viewed as a potential anticancer agent. The ligand and the Pt(II) complex show promising results for further investigation of antiviral activity. PB - Tubitak Scientific & Technical Research Council Turkey, Ankara T2 - Turkish Journal of Biology T1 - Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone VL - 40 IS - 3 SP - 661 EP - 669 DO - 10.3906/biy-1503-23 ER -
@article{ author = "Simic, Vera and Kolarević, Stoimir and Brčeski, Ilija and Jeremić, Dejan and Vuković-Gačić, Branka", year = "2016", abstract = "Metal coordination compounds have an important role in the development of novel drugs. Using the resazurin microtitration assay we assessed the cytotoxicity and antiviral activity of the ligand 2-(diphenylphosphino) benzaldehyde 1-adamantoylhydrazone and its Pd(II) and Pt(II) complexes. Cytotoxicity was tested in A549 human lung adenocarcinoma epithelial cells. We observed that the ligand displayed a more pronounced cytotoxic activity than the platinum-based drug, carboplatin. Morphological evaluation of A549 cells treated with the ligand by acridine orange and ethidium bromide double staining revealed the presence of signs of apoptosis. Antiviral activity against poliovirus type 1 was assessed by examination of the cytopathic effect (CPE) in Hep-2 cells. Cells that were exposed to the 19 mu M ligand before infection displayed a maximal significant reduction (by 24.42 +/- 1.49%) of the CPE. This was likely due to the inhibition of virus receptors and prevention of viral adsorption. Treatment with 17 mu M Pt(II) complex after viral infection caused a maximal significant reduction (by 30.52 +/- 3.12%) of the CPE, presumably through an effect on viral replication. The results indicate that the ligand should be viewed as a potential anticancer agent. The ligand and the Pt(II) complex show promising results for further investigation of antiviral activity.", publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara", journal = "Turkish Journal of Biology", title = "Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone", volume = "40", number = "3", pages = "661-669", doi = "10.3906/biy-1503-23" }
Simic, V., Kolarević, S., Brčeski, I., Jeremić, D.,& Vuković-Gačić, B.. (2016). Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone. in Turkish Journal of Biology Tubitak Scientific & Technical Research Council Turkey, Ankara., 40(3), 661-669. https://doi.org/10.3906/biy-1503-23
Simic V, Kolarević S, Brčeski I, Jeremić D, Vuković-Gačić B. Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone. in Turkish Journal of Biology. 2016;40(3):661-669. doi:10.3906/biy-1503-23 .
Simic, Vera, Kolarević, Stoimir, Brčeski, Ilija, Jeremić, Dejan, Vuković-Gačić, Branka, "Cytotoxicity and antiviral activity of palladium(II) and platinum(II) complexes with 2-(diphenylphosphino)benzaldehyde 1-adamantoylhydrazone" in Turkish Journal of Biology, 40, no. 3 (2016):661-669, https://doi.org/10.3906/biy-1503-23 . .