Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities
Samo za registrovane korisnike
2016
Autori
Stanisavljević, Nemanja S.Ilić, Marija D.
Matić, Ivana Z.
Jovanović, Živko S.
Čupić, Tihomir
Dabić Zagorac, Dragana
Natić, Maja
Tešić, Živoslav Lj.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, an...d myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.
Izvor:
Nutrition and Cancer. An International Journal, 2016, 68, 6, 988-1000Izdavač:
- Routledge Journals, Taylor & Francis Ltd, Abingdon
Finansiranje / projekti:
- Molekularni mehanizmi odgovora biljaka na abiotički stres-uloga transkripcionih faktora i malih RNK i analiza genetičkog diverziteta biljnih kultura od interesa za poljoprivredu i biotehnologiju (RS-173005)
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-172017)
- Modifikatori biološkog odgovora u fiziološkim i patološkim stanjima (RS-175011)
Napomena:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3534
DOI: 10.1080/01635581.2016.1190019
ISSN: 0163-5581
PubMed: 27348025
WoS: 000381276500011
Scopus: 2-s2.0-84976337077
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stanisavljević, Nemanja S. AU - Ilić, Marija D. AU - Matić, Ivana Z. AU - Jovanović, Živko S. AU - Čupić, Tihomir AU - Dabić Zagorac, Dragana AU - Natić, Maja AU - Tešić, Živoslav Lj. PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2291 AB - To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy. PB - Routledge Journals, Taylor & Francis Ltd, Abingdon T2 - Nutrition and Cancer. An International Journal T1 - Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities VL - 68 IS - 6 SP - 988 EP - 1000 DO - 10.1080/01635581.2016.1190019 ER -
@article{ author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj.", year = "2016", abstract = "To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.", publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon", journal = "Nutrition and Cancer. An International Journal", title = "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities", volume = "68", number = "6", pages = "988-1000", doi = "10.1080/01635581.2016.1190019" }
Stanisavljević, N. S., Ilić, M. D., Matić, I. Z., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M.,& Tešić, Ž. Lj.. (2016). Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal Routledge Journals, Taylor & Francis Ltd, Abingdon., 68(6), 988-1000. https://doi.org/10.1080/01635581.2016.1190019
Stanisavljević NS, Ilić MD, Matić IZ, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL. Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal. 2016;68(6):988-1000. doi:10.1080/01635581.2016.1190019 .
Stanisavljević, Nemanja S., Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities" in Nutrition and Cancer. An International Journal, 68, no. 6 (2016):988-1000, https://doi.org/10.1080/01635581.2016.1190019 . .