Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]
Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]
2006
Autori
Bogdanović, JelenaProdanović, Radivoje
Milosavić, Nenad
Prodanović, Olivera
Radotić, Ksenija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount.
Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.
Ključne reči:
antioxidative response / enzyme activity / free radicals / isoelectrofocusingIzvor:
Archives of biological sciences, 2006, 58, 4, 211-214Finansiranje / projekti:
- Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka (RS-MESTD-MPN2006-2010-143043)
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Bogdanović, Jelena AU - Prodanović, Radivoje AU - Milosavić, Nenad AU - Prodanović, Olivera AU - Radotić, Ksenija PY - 2006 UR - https://cherry.chem.bg.ac.rs/handle/123456789/237 AB - Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount. AB - Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji. T2 - Archives of biological sciences T1 - Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně] T1 - Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně] VL - 58 IS - 4 SP - 211 EP - 214 DO - 10.2298/ABS0604211B ER -
@article{ author = "Bogdanović, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija", year = "2006", abstract = "Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount., Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.", journal = "Archives of biological sciences", title = "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně], Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]", volume = "58", number = "4", pages = "211-214", doi = "10.2298/ABS0604211B" }
Bogdanović, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2006). Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of biological sciences, 58(4), 211-214. https://doi.org/10.2298/ABS0604211B
Bogdanović J, Prodanović R, Milosavić N, Prodanović O, Radotić K. Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of biological sciences. 2006;58(4):211-214. doi:10.2298/ABS0604211B .
Bogdanović, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]" in Archives of biological sciences, 58, no. 4 (2006):211-214, https://doi.org/10.2298/ABS0604211B . .