Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production
Abstract
Cell wall invertase (CWI) from Saccharomyces cerevisiae was immobilized in polyacrylamide hydrogels. The aim of the development of a new biocatalyst was to obtain an improved enzyme for invert sugar production. The monomer concentration and enzyme amount in the immobilizate were optimized, and the obtained biocatalyst had an enzyme activity of 138 +/- 6 IU g(-1). The pH and temperature optima were 4.0 and 70 degrees C, respectively. The stability of immobilized enzyme was determined at several temperatures in the absence of substrate and the half-life obtained at 50 degrees C was 81 days, at 60 degrees C, 128 min and at 70 degrees C, 1.24 min. The biocatalyst was tested at low pH values, 3.0 and 3.5 were tested, and showed great stability. The KM values were 34.1 +/- 1.7 and 126.2 +/- 6.3 mM for free and immobilized CWI, respectively. The activation energies were 37.7 and 23.0 kJ mol(-1) for free and immobilized CWI, respectively. Cell wall invertase immobilized in polyacrylamide hydro...gel (CWI-PAA) was tested for the production of high concentrated invert sugar in a batch and a packed bed reactor. After five days of continuous process, the quality and characteristics of the produced invert sugar remained unchanged.
Keywords:
immobilization / Saccharomyces cerevisiae / enzyme / stability / CWI-PAASource:
Journal of the Serbian Chemical Society, 2016, 81, 12, 1359-1369Publisher:
- Serbian Chemical Soc, Belgrade
Funding / projects:
- Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-MESTD-Basic Research (BR or ON)-172048)
DOI: 10.2298/JSC160720094M
ISSN: 0352-5139
WoS: 000392946100003
Scopus: 2-s2.0-85009770680
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Margetić, Aleksandra AU - Vujčić, Zoran PY - 2016 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2390 AB - Cell wall invertase (CWI) from Saccharomyces cerevisiae was immobilized in polyacrylamide hydrogels. The aim of the development of a new biocatalyst was to obtain an improved enzyme for invert sugar production. The monomer concentration and enzyme amount in the immobilizate were optimized, and the obtained biocatalyst had an enzyme activity of 138 +/- 6 IU g(-1). The pH and temperature optima were 4.0 and 70 degrees C, respectively. The stability of immobilized enzyme was determined at several temperatures in the absence of substrate and the half-life obtained at 50 degrees C was 81 days, at 60 degrees C, 128 min and at 70 degrees C, 1.24 min. The biocatalyst was tested at low pH values, 3.0 and 3.5 were tested, and showed great stability. The KM values were 34.1 +/- 1.7 and 126.2 +/- 6.3 mM for free and immobilized CWI, respectively. The activation energies were 37.7 and 23.0 kJ mol(-1) for free and immobilized CWI, respectively. Cell wall invertase immobilized in polyacrylamide hydrogel (CWI-PAA) was tested for the production of high concentrated invert sugar in a batch and a packed bed reactor. After five days of continuous process, the quality and characteristics of the produced invert sugar remained unchanged. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production VL - 81 IS - 12 SP - 1359 EP - 1369 DO - 10.2298/JSC160720094M ER -
@article{ author = "Margetić, Aleksandra and Vujčić, Zoran", year = "2016", abstract = "Cell wall invertase (CWI) from Saccharomyces cerevisiae was immobilized in polyacrylamide hydrogels. The aim of the development of a new biocatalyst was to obtain an improved enzyme for invert sugar production. The monomer concentration and enzyme amount in the immobilizate were optimized, and the obtained biocatalyst had an enzyme activity of 138 +/- 6 IU g(-1). The pH and temperature optima were 4.0 and 70 degrees C, respectively. The stability of immobilized enzyme was determined at several temperatures in the absence of substrate and the half-life obtained at 50 degrees C was 81 days, at 60 degrees C, 128 min and at 70 degrees C, 1.24 min. The biocatalyst was tested at low pH values, 3.0 and 3.5 were tested, and showed great stability. The KM values were 34.1 +/- 1.7 and 126.2 +/- 6.3 mM for free and immobilized CWI, respectively. The activation energies were 37.7 and 23.0 kJ mol(-1) for free and immobilized CWI, respectively. Cell wall invertase immobilized in polyacrylamide hydrogel (CWI-PAA) was tested for the production of high concentrated invert sugar in a batch and a packed bed reactor. After five days of continuous process, the quality and characteristics of the produced invert sugar remained unchanged.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production", volume = "81", number = "12", pages = "1359-1369", doi = "10.2298/JSC160720094M" }
Margetić, A.,& Vujčić, Z.. (2016). Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 81(12), 1359-1369. https://doi.org/10.2298/JSC160720094M
Margetić A, Vujčić Z. Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production. in Journal of the Serbian Chemical Society. 2016;81(12):1359-1369. doi:10.2298/JSC160720094M .
Margetić, Aleksandra, Vujčić, Zoran, "Immobilization of cell wall invertase in a polyacrylamide hydrogel for invert sugar production" in Journal of the Serbian Chemical Society, 81, no. 12 (2016):1359-1369, https://doi.org/10.2298/JSC160720094M . .