Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin
Само за регистроване кориснике
2017
Аутори
Minić, Simeon L.Radomirović, Mirjana Ž.
Radibratović, Milica
Milčić, Miloš K.
Stanić-Vučinić, Dragana
Nikolić, Milan
Ćirković-Veličković, Tanja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Phycocyanobilin (PCB) is a blue tetrapyrrole chromophore of C-phycocyanin, the main protein of the microalga Spirulina, with numerous proven health-related benefits. We examined binding of PCB to bovine serum albumin (BSA) and how it affects protein and ligand stability. Protein fluorescence quenching and microscale thermophoresis demonstrated high-affinity binding (Ka = 2 × 106 M−1). Spectroscopic titration with molecular docking analysis revealed two binding sites on BSA, at the inter-domain cleft and at subdomain IB, while CD spectroscopy indicated stereo-selective binding of the P conformer of the pigment to the protein. The PCB protein complex showed increased thermal stability. Although complex formation partly masked the antioxidant properties of PCB and BSA, a mutually protective effect against free radical-induced oxidation was found. BSA could be suitable for delivery of PCB as a food colorant or bioactive component. Our results also highlight subtle differences between PCB b...inding to bovine vs. human serum albumin.
Кључне речи:
Antioxidant / Binding / Bovine serum albumin / Phycocyanobilin / Spirulina / StabilityИзвор:
FEBS Journal / Federation of European of Biochemical Societies, 2017, 284, 189-190Издавач:
- Wiley, Hoboken
DOI: 10.1016/j.foodchem.2017.07.066
ISSN: 1742-464X
WoS: 000409918902275
Scopus: 2-s2.0-85024374977
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - CONF AU - Minić, Simeon L. AU - Radomirović, Mirjana Ž. AU - Radibratović, Milica AU - Milčić, Miloš K. AU - Stanić-Vučinić, Dragana AU - Nikolić, Milan AU - Ćirković-Veličković, Tanja PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2408 AB - Phycocyanobilin (PCB) is a blue tetrapyrrole chromophore of C-phycocyanin, the main protein of the microalga Spirulina, with numerous proven health-related benefits. We examined binding of PCB to bovine serum albumin (BSA) and how it affects protein and ligand stability. Protein fluorescence quenching and microscale thermophoresis demonstrated high-affinity binding (Ka = 2 × 106 M−1). Spectroscopic titration with molecular docking analysis revealed two binding sites on BSA, at the inter-domain cleft and at subdomain IB, while CD spectroscopy indicated stereo-selective binding of the P conformer of the pigment to the protein. The PCB protein complex showed increased thermal stability. Although complex formation partly masked the antioxidant properties of PCB and BSA, a mutually protective effect against free radical-induced oxidation was found. BSA could be suitable for delivery of PCB as a food colorant or bioactive component. Our results also highlight subtle differences between PCB binding to bovine vs. human serum albumin. PB - Wiley, Hoboken C3 - FEBS Journal / Federation of European of Biochemical Societies T1 - Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin VL - 284 SP - 189 EP - 190 DO - 10.1016/j.foodchem.2017.07.066 ER -
@conference{ author = "Minić, Simeon L. and Radomirović, Mirjana Ž. and Radibratović, Milica and Milčić, Miloš K. and Stanić-Vučinić, Dragana and Nikolić, Milan and Ćirković-Veličković, Tanja", year = "2017", abstract = "Phycocyanobilin (PCB) is a blue tetrapyrrole chromophore of C-phycocyanin, the main protein of the microalga Spirulina, with numerous proven health-related benefits. We examined binding of PCB to bovine serum albumin (BSA) and how it affects protein and ligand stability. Protein fluorescence quenching and microscale thermophoresis demonstrated high-affinity binding (Ka = 2 × 106 M−1). Spectroscopic titration with molecular docking analysis revealed two binding sites on BSA, at the inter-domain cleft and at subdomain IB, while CD spectroscopy indicated stereo-selective binding of the P conformer of the pigment to the protein. The PCB protein complex showed increased thermal stability. Although complex formation partly masked the antioxidant properties of PCB and BSA, a mutually protective effect against free radical-induced oxidation was found. BSA could be suitable for delivery of PCB as a food colorant or bioactive component. Our results also highlight subtle differences between PCB binding to bovine vs. human serum albumin.", publisher = "Wiley, Hoboken", journal = "FEBS Journal / Federation of European of Biochemical Societies", title = "Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin", volume = "284", pages = "189-190", doi = "10.1016/j.foodchem.2017.07.066" }
Minić, S. L., Radomirović, M. Ž., Radibratović, M., Milčić, M. K., Stanić-Vučinić, D., Nikolić, M.,& Ćirković-Veličković, T.. (2017). Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin. in FEBS Journal / Federation of European of Biochemical Societies Wiley, Hoboken., 284, 189-190. https://doi.org/10.1016/j.foodchem.2017.07.066
Minić SL, Radomirović MŽ, Radibratović M, Milčić MK, Stanić-Vučinić D, Nikolić M, Ćirković-Veličković T. Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin. in FEBS Journal / Federation of European of Biochemical Societies. 2017;284:189-190. doi:10.1016/j.foodchem.2017.07.066 .
Minić, Simeon L., Radomirović, Mirjana Ž., Radibratović, Milica, Milčić, Miloš K., Stanić-Vučinić, Dragana, Nikolić, Milan, Ćirković-Veličković, Tanja, "Characterization and effects of binding of food-derived bioactive phycocyanobilin to bovine serum albumin" in FEBS Journal / Federation of European of Biochemical Societies, 284 (2017):189-190, https://doi.org/10.1016/j.foodchem.2017.07.066 . .