Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA
Samo za registrovane korisnike
2017
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity ...toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.
Ključne reči:
differential pulse voltammetry / boron doped diamond electrode / natural alkaloid / DNA / colchicineIzvor:
Electroanalysis, 2017, 29, 10, 2276-2281Izdavač:
- Wiley-V C H Verlag Gmbh, Weinheim
Finansiranje / projekti:
- Primena unapređenih oksidacionih procesa i nanostrukturisanih oksidnih materijala za uklanjanje zagađivača iz životne sredine, razvoj i optimizacija instrumentalnih tehnika za praćenje efikasnosti (RS-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-621375)
- Grant Agency of the Slovak Republic [1/0489/16]
DOI: 10.1002/elan.201700233
ISSN: 1040-0397
WoS: 000412547200014
Scopus: 2-s2.0-85023162696
Kolekcije
Institucija/grupa
Inovacioni centar / Innovation CentreTY - JOUR AU - Stanković, Dalibor AU - Šovrc, Ľubomír AU - Mariano, Jose F. M. L. AU - Ortner, Astrid AU - Kalcher, Kurt PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2534 AB - Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods. PB - Wiley-V C H Verlag Gmbh, Weinheim T2 - Electroanalysis T1 - Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA VL - 29 IS - 10 SP - 2276 EP - 2281 DO - 10.1002/elan.201700233 ER -
@article{ author = "Stanković, Dalibor and Šovrc, Ľubomír and Mariano, Jose F. M. L. and Ortner, Astrid and Kalcher, Kurt", year = "2017", abstract = "Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.", publisher = "Wiley-V C H Verlag Gmbh, Weinheim", journal = "Electroanalysis", title = "Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA", volume = "29", number = "10", pages = "2276-2281", doi = "10.1002/elan.201700233" }
Stanković, D., Šovrc, Ľ., Mariano, J. F. M. L., Ortner, A.,& Kalcher, K.. (2017). Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA. in Electroanalysis Wiley-V C H Verlag Gmbh, Weinheim., 29(10), 2276-2281. https://doi.org/10.1002/elan.201700233
Stanković D, Šovrc Ľ, Mariano JFML, Ortner A, Kalcher K. Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA. in Electroanalysis. 2017;29(10):2276-2281. doi:10.1002/elan.201700233 .
Stanković, Dalibor, Šovrc, Ľubomír, Mariano, Jose F. M. L., Ortner, Astrid, Kalcher, Kurt, "Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA" in Electroanalysis, 29, no. 10 (2017):2276-2281, https://doi.org/10.1002/elan.201700233 . .