Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia
Abstract
In this work a novel hitherto unrecognised minor hemoglobin (Hb) fraction, which we detected previously in hemolysates of erythrocytes exposed to a high concentration of insulin under hypoglycemic conditions, both in vivo and in vitro, is analysed. The modification of Hb in HbA1x was shown to be due the addition of glycoinositolphospholipid (GPI) to the C termini of both beta polypeptide chains. A structurally related minor Hb fraction was identified in erythrocytes exposed in vitro to insulin-mimetic agent, trypsin. To our knowledge this is the first demonstration of such a modification of Hb, as well as the first demonstration of post-translational GPI binding to proteins in response to insulin. The mechanism proposed for GPI-Hb formation is briefly described. (C) 1997 Academic Press.
Source:
Biochemical and Biophysical Research Communications, 1997, 239, 2, 435-438Publisher:
- Academic Press Inc Jnl-Comp Subscriptions, San Diego
DOI: 10.1006/bbrc.1997.7362
ISSN: 0006-291X
PubMed: 9344847
WoS: A1997YD84900013
Scopus: 2-s2.0-0031581053
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Niketic, V AU - Tomasevic, N AU - Nikolić, Milan PY - 1997 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2599 AB - In this work a novel hitherto unrecognised minor hemoglobin (Hb) fraction, which we detected previously in hemolysates of erythrocytes exposed to a high concentration of insulin under hypoglycemic conditions, both in vivo and in vitro, is analysed. The modification of Hb in HbA1x was shown to be due the addition of glycoinositolphospholipid (GPI) to the C termini of both beta polypeptide chains. A structurally related minor Hb fraction was identified in erythrocytes exposed in vitro to insulin-mimetic agent, trypsin. To our knowledge this is the first demonstration of such a modification of Hb, as well as the first demonstration of post-translational GPI binding to proteins in response to insulin. The mechanism proposed for GPI-Hb formation is briefly described. (C) 1997 Academic Press. PB - Academic Press Inc Jnl-Comp Subscriptions, San Diego T2 - Biochemical and Biophysical Research Communications T1 - Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia VL - 239 IS - 2 SP - 435 EP - 438 DO - 10.1006/bbrc.1997.7362 ER -
@article{ author = "Niketic, V and Tomasevic, N and Nikolić, Milan", year = "1997", abstract = "In this work a novel hitherto unrecognised minor hemoglobin (Hb) fraction, which we detected previously in hemolysates of erythrocytes exposed to a high concentration of insulin under hypoglycemic conditions, both in vivo and in vitro, is analysed. The modification of Hb in HbA1x was shown to be due the addition of glycoinositolphospholipid (GPI) to the C termini of both beta polypeptide chains. A structurally related minor Hb fraction was identified in erythrocytes exposed in vitro to insulin-mimetic agent, trypsin. To our knowledge this is the first demonstration of such a modification of Hb, as well as the first demonstration of post-translational GPI binding to proteins in response to insulin. The mechanism proposed for GPI-Hb formation is briefly described. (C) 1997 Academic Press.", publisher = "Academic Press Inc Jnl-Comp Subscriptions, San Diego", journal = "Biochemical and Biophysical Research Communications", title = "Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia", volume = "239", number = "2", pages = "435-438", doi = "10.1006/bbrc.1997.7362" }
Niketic, V., Tomasevic, N.,& Nikolić, M.. (1997). Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia. in Biochemical and Biophysical Research Communications Academic Press Inc Jnl-Comp Subscriptions, San Diego., 239(2), 435-438. https://doi.org/10.1006/bbrc.1997.7362
Niketic V, Tomasevic N, Nikolić M. Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia. in Biochemical and Biophysical Research Communications. 1997;239(2):435-438. doi:10.1006/bbrc.1997.7362 .
Niketic, V, Tomasevic, N, Nikolić, Milan, "Covalent glycoinositolphospholipid binding to hemoglobin: A new post-translational modification of hb occurring in hyperinsulinism with concomitant hypoglycemia" in Biochemical and Biophysical Research Communications, 239, no. 2 (1997):435-438, https://doi.org/10.1006/bbrc.1997.7362 . .