High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples
Аутори
Ristivojević, PetarTahir, Ammar
Malfent, Fabian
Milojković-Opsenica, Dušanka
Rollinger, Judith M.
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Quality control is a crucial step in the production of effective and safe herbal remedies. The aim of this study was to develop a new, simple, and high throughput procedure for the quality assessment of herbal drugs using a high-performance thin-layer chromatography (HPTLC)/bioautography and UPLC–MS/MS approach combined with chemometrics. This was exemplarily shown for Morus alba L. root bark (sāng bái pí; SBP). Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, which was supported by UPLC–MS/MS analysis. Further, the combination of bioautography and chemometrics identified those samples with the most bioactive constituents. Plant materials collected from Serbia (11 samples) showed higher both radical scavenging and antimicrobial activities compared to samples provided from China (7 samples). Principal component analysis (PCA) ...confirmed the discrimination of geographically different samples and recognized their main markers responsible for differences between Serbian and Chinese samples. Most importantly for quality assessment, the combined HPTLC/bioautography and UPLC–MS/MS approach not only allowed for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP.
Кључне речи:
Bioautography / HPTLC / Pattern recognition / Quality control / sāng bái pí / UPLC–MS/MSИзвор:
Journal of Chromatography A, 2019, 1594, 190-198Издавач:
- Elsevier
Финансирање / пројекти:
- Корелација структуре и особина природних и синтетичких молекула и њихових комплекса са металима (RS-MESTD-Basic Research (BR or ON)-172017)
Напомена:
- Supplementary material : http://cherry.chem.bg.ac.rs/handle/123456789/2919
- This is the peer-reviewed version of the following article:Ristivojević, P.; Tahir, A.; Malfent, F.; Milojković-Opsenica, D.; Rollinger, J. M. High-Performance Thin-Layer Chromatography/Bioautography and Liquid Chromatography-Mass Spectrometry Hyphenated with Chemometrics for the Quality Assessment of Morus Alba Samples. Journal of Chromatography A 2019, 1594, 190–198. https://doi.org/10.1016/j.chroma.2019.02.006
DOI: 10.1016/j.chroma.2019.02.006
ISSN: 0021-9673
WoS: 000467665400020
Scopus: 2-s2.0-85061118029
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ristivojević, Petar AU - Tahir, Ammar AU - Malfent, Fabian AU - Milojković-Opsenica, Dušanka AU - Rollinger, Judith M. PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2944 AB - Quality control is a crucial step in the production of effective and safe herbal remedies. The aim of this study was to develop a new, simple, and high throughput procedure for the quality assessment of herbal drugs using a high-performance thin-layer chromatography (HPTLC)/bioautography and UPLC–MS/MS approach combined with chemometrics. This was exemplarily shown for Morus alba L. root bark (sāng bái pí; SBP). Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, which was supported by UPLC–MS/MS analysis. Further, the combination of bioautography and chemometrics identified those samples with the most bioactive constituents. Plant materials collected from Serbia (11 samples) showed higher both radical scavenging and antimicrobial activities compared to samples provided from China (7 samples). Principal component analysis (PCA) confirmed the discrimination of geographically different samples and recognized their main markers responsible for differences between Serbian and Chinese samples. Most importantly for quality assessment, the combined HPTLC/bioautography and UPLC–MS/MS approach not only allowed for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP. PB - Elsevier T2 - Journal of Chromatography A T1 - High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples VL - 1594 SP - 190 EP - 198 DO - 10.1016/j.chroma.2019.02.006 ER -
@article{ author = "Ristivojević, Petar and Tahir, Ammar and Malfent, Fabian and Milojković-Opsenica, Dušanka and Rollinger, Judith M.", year = "2019", abstract = "Quality control is a crucial step in the production of effective and safe herbal remedies. The aim of this study was to develop a new, simple, and high throughput procedure for the quality assessment of herbal drugs using a high-performance thin-layer chromatography (HPTLC)/bioautography and UPLC–MS/MS approach combined with chemometrics. This was exemplarily shown for Morus alba L. root bark (sāng bái pí; SBP). Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, which was supported by UPLC–MS/MS analysis. Further, the combination of bioautography and chemometrics identified those samples with the most bioactive constituents. Plant materials collected from Serbia (11 samples) showed higher both radical scavenging and antimicrobial activities compared to samples provided from China (7 samples). Principal component analysis (PCA) confirmed the discrimination of geographically different samples and recognized their main markers responsible for differences between Serbian and Chinese samples. Most importantly for quality assessment, the combined HPTLC/bioautography and UPLC–MS/MS approach not only allowed for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP.", publisher = "Elsevier", journal = "Journal of Chromatography A", title = "High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples", volume = "1594", pages = "190-198", doi = "10.1016/j.chroma.2019.02.006" }
Ristivojević, P., Tahir, A., Malfent, F., Milojković-Opsenica, D.,& Rollinger, J. M.. (2019). High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples. in Journal of Chromatography A Elsevier., 1594, 190-198. https://doi.org/10.1016/j.chroma.2019.02.006
Ristivojević P, Tahir A, Malfent F, Milojković-Opsenica D, Rollinger JM. High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples. in Journal of Chromatography A. 2019;1594:190-198. doi:10.1016/j.chroma.2019.02.006 .
Ristivojević, Petar, Tahir, Ammar, Malfent, Fabian, Milojković-Opsenica, Dušanka, Rollinger, Judith M., "High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples" in Journal of Chromatography A, 1594 (2019):190-198, https://doi.org/10.1016/j.chroma.2019.02.006 . .