Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis
Samo za registrovane korisnike
2019
Autori
Stanković, M.Bartolić, D.
Šikoparija, Branko
Spasojević, Dragica
Mutavdžić, Dragosav
Natić, Maja
Radotić, Ksenija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 fo...r (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.
Ključne reči:
fluorescence spectroscopy / honey / melissopalynological analysis / multivariate curve resolution alternating least squares / phenol and protein quantification / ratio metric indicatorIzvor:
Journal of Applied Spectroscopy, 2019, 86, 2, 256-263Finansiranje / projekti:
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-173017)
- Konzervaciona strategija za očuvanje zaštićenih i strogo zaštićenih vrsta u Srbiji - osolike muve (Insecta: Diptera: Syrphidae) kao model organizmi (RS-173002)
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-172017)
DOI: 10.1007/s10812-019-00809-1
ISSN: 0021-9037
WoS: 000468034300010
Scopus: 2-s2.0-85065559772
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stanković, M. AU - Bartolić, D. AU - Šikoparija, Branko AU - Spasojević, Dragica AU - Mutavdžić, Dragosav AU - Natić, Maja AU - Radotić, Ksenija PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3115 AB - It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased. T2 - Journal of Applied Spectroscopy T1 - Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis VL - 86 IS - 2 SP - 256 EP - 263 DO - 10.1007/s10812-019-00809-1 ER -
@article{ author = "Stanković, M. and Bartolić, D. and Šikoparija, Branko and Spasojević, Dragica and Mutavdžić, Dragosav and Natić, Maja and Radotić, Ksenija", year = "2019", abstract = "It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.", journal = "Journal of Applied Spectroscopy", title = "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis", volume = "86", number = "2", pages = "256-263", doi = "10.1007/s10812-019-00809-1" }
Stanković, M., Bartolić, D., Šikoparija, B., Spasojević, D., Mutavdžić, D., Natić, M.,& Radotić, K.. (2019). Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. in Journal of Applied Spectroscopy, 86(2), 256-263. https://doi.org/10.1007/s10812-019-00809-1
Stanković M, Bartolić D, Šikoparija B, Spasojević D, Mutavdžić D, Natić M, Radotić K. Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. in Journal of Applied Spectroscopy. 2019;86(2):256-263. doi:10.1007/s10812-019-00809-1 .
Stanković, M., Bartolić, D., Šikoparija, Branko, Spasojević, Dragica, Mutavdžić, Dragosav, Natić, Maja, Radotić, Ksenija, "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis" in Journal of Applied Spectroscopy, 86, no. 2 (2019):256-263, https://doi.org/10.1007/s10812-019-00809-1 . .