High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents
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A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method was applied in combination with powerful pattern recognition techniques for differentiating thickening agents, which are mainly based on polysaccharides or biopolymers. After methanolysis, the monomeric units of the thickeners were separated by HPTLC and detected using derivatization with the aniline diphenylamine o-phosphoric acid reagent. According to their resulting fingerprint and chemical pattern, the thickening agents studied have been classified by principal component analysis and by hierarchic cluster analysis in several groups. This newly combined approach using HPTLC fingerprints and pattern recognition techniques differentiated high similarity thickeners. Monomeric units responsible for the classification of the investigated thickener have been identified. The results showed that the HPTLC technique in combination with chemometrics can be a very reliable technique for authentication of hi...gh similarity thickening agents and can be used for a quick screening of additives in foodstuffs.
Keywords:
Biopolymers / High-performance thin-layer chromatography / HPTLC fingerprint / Pattern recognition / Polysaccharides / ThickenersSource:
Food Hydrocolloids, 2017, 64, 78-84Publisher:
- Elsevier Sci Ltd, Oxford
Note:
- This is the peer-reviewed version of the following article: Ristivojević, P.; Morlock, G. E. High-Performance Thin-Layer Chromatography Combined with Pattern Recognition Techniques as Tool to Distinguish Thickening Agents. Food Hydrocolloids 2017, 64, 78–84. https://doi.org/10.1016/j.foodhyd.2016.10.005
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3218
DOI: 10.1016/j.foodhyd.2016.10.005
ISSN: 0268-005X
WoS: 000390721500009
Scopus: 2-s2.0-84994057012
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Inovacioni centar / Innovation CentreTY - JOUR AU - Ristivojević, Petar AU - Morlock, Gertrud E. PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3217 AB - A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method was applied in combination with powerful pattern recognition techniques for differentiating thickening agents, which are mainly based on polysaccharides or biopolymers. After methanolysis, the monomeric units of the thickeners were separated by HPTLC and detected using derivatization with the aniline diphenylamine o-phosphoric acid reagent. According to their resulting fingerprint and chemical pattern, the thickening agents studied have been classified by principal component analysis and by hierarchic cluster analysis in several groups. This newly combined approach using HPTLC fingerprints and pattern recognition techniques differentiated high similarity thickeners. Monomeric units responsible for the classification of the investigated thickener have been identified. The results showed that the HPTLC technique in combination with chemometrics can be a very reliable technique for authentication of high similarity thickening agents and can be used for a quick screening of additives in foodstuffs. PB - Elsevier Sci Ltd, Oxford T2 - Food Hydrocolloids T1 - High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents VL - 64 SP - 78 EP - 84 DO - 10.1016/j.foodhyd.2016.10.005 ER -
@article{ author = "Ristivojević, Petar and Morlock, Gertrud E.", year = "2017", abstract = "A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method was applied in combination with powerful pattern recognition techniques for differentiating thickening agents, which are mainly based on polysaccharides or biopolymers. After methanolysis, the monomeric units of the thickeners were separated by HPTLC and detected using derivatization with the aniline diphenylamine o-phosphoric acid reagent. According to their resulting fingerprint and chemical pattern, the thickening agents studied have been classified by principal component analysis and by hierarchic cluster analysis in several groups. This newly combined approach using HPTLC fingerprints and pattern recognition techniques differentiated high similarity thickeners. Monomeric units responsible for the classification of the investigated thickener have been identified. The results showed that the HPTLC technique in combination with chemometrics can be a very reliable technique for authentication of high similarity thickening agents and can be used for a quick screening of additives in foodstuffs.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Hydrocolloids", title = "High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents", volume = "64", pages = "78-84", doi = "10.1016/j.foodhyd.2016.10.005" }
Ristivojević, P.,& Morlock, G. E.. (2017). High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents. in Food Hydrocolloids Elsevier Sci Ltd, Oxford., 64, 78-84. https://doi.org/10.1016/j.foodhyd.2016.10.005
Ristivojević P, Morlock GE. High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents. in Food Hydrocolloids. 2017;64:78-84. doi:10.1016/j.foodhyd.2016.10.005 .
Ristivojević, Petar, Morlock, Gertrud E., "High-performance thin-layer chromatography combined with pattern recognition techniques as tool to distinguish thickening agents" in Food Hydrocolloids, 64 (2017):78-84, https://doi.org/10.1016/j.foodhyd.2016.10.005 . .