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Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina
dc.creator | Minić, Simeon L. | |
dc.creator | Stanić-Vučinić, Dragana | |
dc.creator | Mihailović-Vesić, Jelena | |
dc.creator | Krstić-Ristivojević, Maja | |
dc.creator | Nikolić, Milan | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.date.accessioned | 2019-09-19T08:07:15Z | |
dc.date.available | 2017-03-28 | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1874-3919 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/3444 | |
dc.description.abstract | C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved. | en |
dc.publisher | Elsevier Science Bv, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS// | |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/256716/EU// | |
dc.rights | embargoedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of Proteomics | |
dc.subject | C-phycocyanin | en |
dc.subject | Phycocyanobilin | en |
dc.subject | Digestibility | en |
dc.subject | Peptides | en |
dc.subject | Bioactivity | en |
dc.title | Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Станић-Вучинић, Драгана; Михаиловић-Весић, Јелена; Николић, Милан; Ћирковић-Величковић, Тања; Крстић-Ристивојевић, Маја; Минић, Симеон Л.; | |
dc.citation.volume | 147 | |
dc.citation.spage | 132 | |
dc.citation.epage | 139 | |
dc.identifier.wos | 000383942500015 | |
dc.identifier.doi | 10.1016/j.jprot.2016.03.043 | |
dc.citation.other | 147: 132-139 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 27084687 | |
dc.description.other | This is peer-reviewed version of the following article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. [https://doi.org/10.1016/j.jprot.2016.03.043] | |
dc.description.other | Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3445] | |
dc.type.version | acceptedVersion | en |
dc.identifier.scopus | 2-s2.0-84963972252 | |
dc.identifier.fulltext | https://cherry.chem.bg.ac.rs/bitstream/id/15032/Digestion_by_pepsin_acc_2016.pdf |