Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
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2019
Authors
Nešić, Andrijana N.Stam, Annemarie
Čavić, Milena
Ten Klooster, Jean Paul
Pieters, Raymond
Smit, Joost
Gavrović-Jankulović, Marija
Article (Published version)
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In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.
Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.
2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.
Keywords:
Act d 1 / Cysteine protease / Food allergy / Pro-inflammatory cytokines / TJ proteinsSource:
Journal of Functional Foods, 2019, 62Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
- Institute for Risk Assessment Sciences, University of Utrecht,Netherlands, which was supported by COST Action FA1402.
DOI: 10.1016/j.jff.2019.103556
ISSN: 1756-4646
WoS: 000491207500030
Scopus: 2-s2.0-85071845404
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Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Nešić, Andrijana N. AU - Stam, Annemarie AU - Čavić, Milena AU - Ten Klooster, Jean Paul AU - Pieters, Raymond AU - Smit, Joost AU - Gavrović-Jankulović, Marija PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3485 AB - In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids. Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1. 2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins. T2 - Journal of Functional Foods T1 - Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model VL - 62 DO - 10.1016/j.jff.2019.103556 ER -
@article{ author = "Nešić, Andrijana N. and Stam, Annemarie and Čavić, Milena and Ten Klooster, Jean Paul and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija", year = "2019", abstract = "In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids. Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1. 2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.", journal = "Journal of Functional Foods", title = "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model", volume = "62", doi = "10.1016/j.jff.2019.103556" }
Nešić, A. N., Stam, A., Čavić, M., Ten Klooster, J. P., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods, 62. https://doi.org/10.1016/j.jff.2019.103556
Nešić AN, Stam A, Čavić M, Ten Klooster JP, Pieters R, Smit J, Gavrović-Jankulović M. Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods. 2019;62. doi:10.1016/j.jff.2019.103556 .
Nešić, Andrijana N., Stam, Annemarie, Čavić, Milena, Ten Klooster, Jean Paul, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model" in Journal of Functional Foods, 62 (2019), https://doi.org/10.1016/j.jff.2019.103556 . .