Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
Аутори
Stanković, NadaŠenerović, Lidija
Bojić-Trbojević, Žanka
Vučković, Ivan
Vicovac, Ljiljana
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less t...oxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
Кључне речи:
actinomycetes / antimicrobials / cytotoxicity / fungi / optimizationИзвор:
Journal of Applied Microbiology, 2013, 115, 6, 1297-1306Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-173048)
- Трофобласт и екстраембрионалне феталне ћелије: пластичност, фактори диференцијације и ин витро модулација функционалних својстава (RS-173004)
Напомена:
- This is the peer-reviewed version of the following article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3487
DOI: 10.1111/jam.12326
ISSN: 1364-5072
WoS: 000326939800005
Scopus: 2-s2.0-84887823790
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stanković, Nada AU - Šenerović, Lidija AU - Bojić-Trbojević, Žanka AU - Vučković, Ivan AU - Vicovac, Ljiljana AU - Vasiljević, Branka AU - Nikodinović-Runić, Jasmina PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3486 AB - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant. PB - Wiley, Hoboken T2 - Journal of Applied Microbiology T1 - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity VL - 115 IS - 6 SP - 1297 EP - 1306 DO - 10.1111/jam.12326 ER -
@article{ author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Žanka and Vučković, Ivan and Vicovac, Ljiljana and Vasiljević, Branka and Nikodinović-Runić, Jasmina", year = "2013", abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.", publisher = "Wiley, Hoboken", journal = "Journal of Applied Microbiology", title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity", volume = "115", number = "6", pages = "1297-1306", doi = "10.1111/jam.12326" }
Stanković, N., Šenerović, L., Bojić-Trbojević, Ž., Vučković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology Wiley, Hoboken., 115(6), 1297-1306. https://doi.org/10.1111/jam.12326
Stanković N, Šenerović L, Bojić-Trbojević Ž, Vučković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306. doi:10.1111/jam.12326 .
Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Žanka, Vučković, Ivan, Vicovac, Ljiljana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306, https://doi.org/10.1111/jam.12326 . .