Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2
Authors
Pompach, PetrViola, Cristina M.
Radosavljević, Jelena
Lin, Jingjing
Jiráček, Jirˇí
Brzozowski, Andrzej M.
Selicharová, Irena
Article (Published version)
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Show full item recordAbstract
Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques and reveal details of these protein-protein interfaces. We used such approach to clarify missing structural description of the interface in human insulin-like growth factor (IGF-1): Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray crystallography. We crosslinked these proteins by heterobifunctional cross-linker sulfosuccinimidyl 4,4′-azidopentanoate (Sulfo-SDA) for the subsequent mass spectrometry (MS) analysis. The MS analysis revealed IGF-1:Imp-L2 interactions which were not resolved in the crystal structure of this assembly, and they converged with X-ray results, indicating the importance of the IGF-1 N-terminus interaction with the C-terminal (185–242) part of the Imp-L2 for stability of this comple...x. Here, we also showed the advantage and reliability of MS approach in solving details of protein-protein interactions that are too flexible for solid state structural methods.
Keywords:
cross-linking / diazirine ring / IGF-1 / Imp-L2 / mass spectrometrySource:
Frontiers in Endocrinology, 2019, 10Publisher:
- Frontiers Media S.A.
Funding / projects:
- Medical Research Council Grants MR/K000179/1 and MR/R009066/1
- Chemical biology for drugging undruggable targets (ChemBioDrug) (No. CZ.02.1.01/0.0/0.0/16_019/0000729)
- Czech Infrastructure for Integrative Structural Biology (LM2015043 CIISB for CMS BIOCEV funded by MEYS CR)
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3720
DOI: 10.3389/fendo.2019.00695
ISSN: 1664-2392
WoS: 000497451900001
Scopus: 2-s2.0-85074154541
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Pompach, Petr AU - Viola, Cristina M. AU - Radosavljević, Jelena AU - Lin, Jingjing AU - Jiráček, Jirˇí AU - Brzozowski, Andrzej M. AU - Selicharová, Irena PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3719 AB - Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques and reveal details of these protein-protein interfaces. We used such approach to clarify missing structural description of the interface in human insulin-like growth factor (IGF-1): Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray crystallography. We crosslinked these proteins by heterobifunctional cross-linker sulfosuccinimidyl 4,4′-azidopentanoate (Sulfo-SDA) for the subsequent mass spectrometry (MS) analysis. The MS analysis revealed IGF-1:Imp-L2 interactions which were not resolved in the crystal structure of this assembly, and they converged with X-ray results, indicating the importance of the IGF-1 N-terminus interaction with the C-terminal (185–242) part of the Imp-L2 for stability of this complex. Here, we also showed the advantage and reliability of MS approach in solving details of protein-protein interactions that are too flexible for solid state structural methods. PB - Frontiers Media S.A. T2 - Frontiers in Endocrinology T1 - Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2 VL - 10 DO - 10.3389/fendo.2019.00695 ER -
@article{ author = "Pompach, Petr and Viola, Cristina M. and Radosavljević, Jelena and Lin, Jingjing and Jiráček, Jirˇí and Brzozowski, Andrzej M. and Selicharová, Irena", year = "2019", abstract = "Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques and reveal details of these protein-protein interfaces. We used such approach to clarify missing structural description of the interface in human insulin-like growth factor (IGF-1): Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray crystallography. We crosslinked these proteins by heterobifunctional cross-linker sulfosuccinimidyl 4,4′-azidopentanoate (Sulfo-SDA) for the subsequent mass spectrometry (MS) analysis. The MS analysis revealed IGF-1:Imp-L2 interactions which were not resolved in the crystal structure of this assembly, and they converged with X-ray results, indicating the importance of the IGF-1 N-terminus interaction with the C-terminal (185–242) part of the Imp-L2 for stability of this complex. Here, we also showed the advantage and reliability of MS approach in solving details of protein-protein interactions that are too flexible for solid state structural methods.", publisher = "Frontiers Media S.A.", journal = "Frontiers in Endocrinology", title = "Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2", volume = "10", doi = "10.3389/fendo.2019.00695" }
Pompach, P., Viola, C. M., Radosavljević, J., Lin, J., Jiráček, J., Brzozowski, A. M.,& Selicharová, I.. (2019). Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. in Frontiers in Endocrinology Frontiers Media S.A.., 10. https://doi.org/10.3389/fendo.2019.00695
Pompach P, Viola CM, Radosavljević J, Lin J, Jiráček J, Brzozowski AM, Selicharová I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. in Frontiers in Endocrinology. 2019;10. doi:10.3389/fendo.2019.00695 .
Pompach, Petr, Viola, Cristina M., Radosavljević, Jelena, Lin, Jingjing, Jiráček, Jirˇí, Brzozowski, Andrzej M., Selicharová, Irena, "Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2" in Frontiers in Endocrinology, 10 (2019), https://doi.org/10.3389/fendo.2019.00695 . .