Antitumour effect of a mixture of n-propyl polysulfides In vitro
Authors
Đorđević, Dragana S.Milovanović, Jelena
Jurisević, Milena
Stojanović, Bojana
Cvetković, Olga
Pergal, Marija V.
Ristanović, Elizabeta
Vojvodić, Danilo
Simić, Miloš
Manojlović, Dragan D.
Milovanović, Marija
Arsenijević, Nebojša
Article (Published version)
Metadata
Show full item recordAbstract
Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays.
The results ...of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.
Keywords:
Cytotoxicity / In vitro / N-propyl polysulfides / Tumour cell linesSource:
Serbian Journal of Experimental and Clinical Research, 2019, 20, 4, 295-300Funding / projects:
- Interleukin 33 /ST2 axis and galectin-3 in the pathogenesis of experimental periapical lesions. (RS-MESTD-Basic Research (BR or ON)-175071)
- Molecular determinants of innate immunity in autoimmunity and tumorogenesis (RS-MESTD-Basic Research (BR or ON)-175069)
- Developing infrastructure for priority research fields (RS-MESTD-Basic Research (BR or ON)-175103)
- The Faculty of Medical Sciences, University of Kragujevac (MP 01/14, MP 02/14, and JP 24/10).
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Đorđević, Dragana S. AU - Milovanović, Jelena AU - Jurisević, Milena AU - Stojanović, Bojana AU - Cvetković, Olga AU - Pergal, Marija V. AU - Ristanović, Elizabeta AU - Vojvodić, Danilo AU - Simić, Miloš AU - Manojlović, Dragan D. AU - Milovanović, Marija AU - Arsenijević, Nebojša PY - 2019 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3858 AB - Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays. The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin. T2 - Serbian Journal of Experimental and Clinical Research T1 - Antitumour effect of a mixture of n-propyl polysulfides In vitro VL - 20 IS - 4 SP - 295 EP - 300 DO - 10.1515/sjecr-2017-0069 ER -
@article{ author = "Đorđević, Dragana S. and Milovanović, Jelena and Jurisević, Milena and Stojanović, Bojana and Cvetković, Olga and Pergal, Marija V. and Ristanović, Elizabeta and Vojvodić, Danilo and Simić, Miloš and Manojlović, Dragan D. and Milovanović, Marija and Arsenijević, Nebojša", year = "2019", abstract = "Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays. The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.", journal = "Serbian Journal of Experimental and Clinical Research", title = "Antitumour effect of a mixture of n-propyl polysulfides In vitro", volume = "20", number = "4", pages = "295-300", doi = "10.1515/sjecr-2017-0069" }
Đorđević, D. S., Milovanović, J., Jurisević, M., Stojanović, B., Cvetković, O., Pergal, M. V., Ristanović, E., Vojvodić, D., Simić, M., Manojlović, D. D., Milovanović, M.,& Arsenijević, N.. (2019). Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research, 20(4), 295-300. https://doi.org/10.1515/sjecr-2017-0069
Đorđević DS, Milovanović J, Jurisević M, Stojanović B, Cvetković O, Pergal MV, Ristanović E, Vojvodić D, Simić M, Manojlović DD, Milovanović M, Arsenijević N. Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research. 2019;20(4):295-300. doi:10.1515/sjecr-2017-0069 .
Đorđević, Dragana S., Milovanović, Jelena, Jurisević, Milena, Stojanović, Bojana, Cvetković, Olga, Pergal, Marija V., Ristanović, Elizabeta, Vojvodić, Danilo, Simić, Miloš, Manojlović, Dragan D., Milovanović, Marija, Arsenijević, Nebojša, "Antitumour effect of a mixture of n-propyl polysulfides In vitro" in Serbian Journal of Experimental and Clinical Research, 20, no. 4 (2019):295-300, https://doi.org/10.1515/sjecr-2017-0069 . .